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Making statistics intuitive

Test Statistic: Definition, Types & Formulas

By Jim Frost 10 Comments

What is a Test Statistic?

A test statistic assesses how consistent your sample data are with the null hypothesis in a hypothesis test. Test statistic calculations take your sample data and boil them down to a single number that quantifies how much your sample diverges from the null hypothesis. As a test statistic value becomes more extreme, it indicates larger differences between your sample data and the null hypothesis.

When your test statistic indicates a sufficiently large incompatibility with the null hypothesis, you can reject the null and state that your results are statistically significant—your data support the notion that the sample effect exists in the population . To use a test statistic to evaluate statistical significance, you either compare it to a critical value or use it to calculate the p-value .

Statisticians named the hypothesis tests after the test statistics because they’re the quantity that the tests actually evaluate. For example, t-tests assess t-values, F-tests evaluate F-values, and chi-square tests use, you guessed it, chi-square values.

In this post, learn about test statistics, how to calculate them, interpret them, and evaluate statistical significance using the critical value and p-value methods.

How to Find Test Statistics

Each test statistic has its own formula. I present several common test statistics examples below. To see worked examples for each one, click the links to my more detailed articles.

Formulas for Test Statistics


T-value for 1-sample t-test		Take the sample mean, subtract the hypothesized mean, and divide by the .
T-value for 2-sample t-test		Take one sample mean, subtract the other, and divide by the pooled standard deviation.
F-value for F-tests and ANOVA		Calculate the ratio of two .
Chi-squared value (χ ) for a Chi-squared test		Sum the squared differences between observed and expected values divided by the expected values.

Understanding the Null Values and the Test Statistic Formulas

In the formulas above, it’s helpful to understand the null condition and the test statistic value that occurs when your sample data match that condition exactly. Also, it’s worthwhile knowing what causes the test statistics to move further away from the null value, potentially becoming significant. Test statistics are statistically significant when they exceed a critical value.

All these test statistics are ratios, which helps you understand their null values.

T-Tests, Null = 0

When a t-value equals 0, it indicates that your sample data match the null hypothesis exactly.

For a 1-sample t-test, when the sample mean equals the hypothesized mean, the numerator is zero, which causes the entire t-value ratio to equal zero. As the sample mean moves away from the hypothesized mean in either the positive or negative direction, the test statistic moves away from zero in the same direction.

A similar case exists for 2-sample t-tests. When the two sample means are equal, the numerator is zero, and the entire test statistic ratio is zero. As the two sample means become increasingly different, the absolute value of the numerator increases, and the t-value becomes more positive or negative.

F-tests including ANOVA, Null = 1

When an F-value equals 1, it indicates that the two variances in the numerator and denominator are equal, matching the null hypothesis.

As the numerator and denominator become less and less similar, the F-value moves away from one in either direction.

Chi-squared Tests, Null = 0

When a chi-squared value equals 0, it indicates that the observed values always match the expected values. This condition causes the numerator to equal zero, making the chi-squared value equal zero.

As the observed values progressively fail to match the expected values, the numerator increases, causing the test statistic to rise from zero.

Related post : How a Chi-Squared Test Works

You’ll never see a test statistic that equals the null value precisely in practice. However, trivial differences been sample values and the null value are not uncommon.

Interpreting Test Statistics

Test statistics are unitless. This fact can make them difficult to interpret on their own. You know they evaluate how well your data agree with the null hypothesis. If your test statistic is extreme enough, your data are so incompatible with the null hypothesis that you can reject it and conclude that your results are statistically significant. But how does that translate to specific values of your test statistic? Where do you draw the line?

For instance, t-values of zero match the null value. But how far from zero should your t-value be to be statistically significant? Is 1 enough? 2? 3? If your t-value is 2, what does it mean anyway? In this case, we know that the sample mean doesn’t equal the null value, but how exceptional is it? To complicate matters, the dividing line changes depending on your sample size and other study design issues.

Similar types of questions apply to the other test statistics too.

To interpret individual values of a test statistic, we need to place them in a larger context. Towards this end, let me introduce you to sampling distributions for test statistics!

Sampling Distributions for Test Statistics

Performing a hypothesis test on a sample produces a single test statistic. Now, imagine you carry out the following process:

Assume the null hypothesis is true in the population.
Repeat your study many times by drawing many random samples of the same size from this population.
Perform the same hypothesis test on all these samples and save the test statistics.
Plot the distribution of the test statistics.

This process produces the distribution of test statistic values that occurs when the effect does not exist in the population (i.e., the null hypothesis is true). Statisticians refer to this type of distribution as a sampling distribution, a kind of probability distribution.

Why would we need this type of distribution?

It provides the larger context required for interpreting a test statistic. More specifically, it allows us to compare our study’s single test statistic to values likely to occur when the null is true. We can quantify our sample statistic’s rareness while assuming the effect does not exist in the population. Now that’s helpful!

Fortunately, we don’t need to collect many random samples to create this distribution! Statisticians have developed formulas allowing us to estimate sampling distributions for test statistics using the sample data.

To evaluate your data’s compatibility with the null hypothesis, place your study’s test statistic in the distribution.

Related post : Understanding Probability Distributions

Example of a Test Statistic in a Sampling Distribution

Suppose our t-test produces a t-value of two. That’s our test statistic. Let’s see where it fits in.

The sampling distribution below shows a t-distribution with 20 degrees of freedom, equating to a 1-sample t-test with a sample size of 21. The distribution centers on zero because it assumes the null hypothesis is correct. When the null is true, your analysis is most likely to obtain a t-value near zero and less likely to produce t-values further from zero in either direction.

Sampling distribution for the t-value test statistic.

The sampling distribution indicates that our test statistic is somewhat rare when we assume the null hypothesis is correct. However, the chances of observing t-values from -2 to +2 are not totally inconceivable. We need a way to quantify the likelihood.

From this point, we need to use the sampling distributions’ ability to calculate probabilities for test statistics.

Related post : Sampling Distributions Explained

Test Statistics and Critical Values

The significance level uses critical values to define how far the test statistic must be from the null value to reject the null hypothesis. When the test statistic exceeds a critical value, the results are statistically significant.

The percentage of the area beneath the sampling distribution curve that is shaded represents the probability that the test statistic will fall in those regions when the null is true. Consequently, to depict a significance level of 0.05, I’ll shade 5% of the sampling distribution furthest away from the null value.

The two shaded areas are equidistant from the null value in the center. Each region has a likelihood of 0.025, which sums to our significance level of 0.05. These shaded areas are the critical regions for a two-tailed hypothesis test. Let’s return to our example t-value of 2.

Related post : What are Critical Values?

Sampling distribution that displays the critical values for our t-value.

In this example, the critical values are -2.086 and +2.086. Our test statistic of 2 is not statistically significant because it does not exceed the critical value.

Other hypothesis tests have their own test statistics and sampling distributions, but their processes for critical values are generally similar.

Learn how to find critical values for test statistics using tables:

T-distribution table
Chi-square table

Related post : Understanding Significance Levels

Using Test Statistics to Find P-values

P-values are the probability of observing an effect at least as extreme as your sample’s effect if you assume no effect exists in the population.

Test statistics represent effect sizes in hypothesis tests because they denote the difference between your sample effect and no effect —the null hypothesis. Consequently, you use the test statistic to calculate the p-value for your hypothesis test.

The above p-value definition is a bit tortuous. Fortunately, it’s much easier to understand how test statistics and p-values work together using a sampling distribution graph.

Let’s use our hypothetical test statistic t-value of 2 for this example. However, because I’m displaying the results of a two-tailed test, I need to use t-values of +2 and -2 to cover both tails.

Related post : One-tailed vs. Two-Tailed Hypothesis Tests

The graph below displays the probability of t-values less than -2 and greater than +2 using the area under the curve. This graph is specific to our t-test design (1-sample t-test with N = 21).

Graph of t-distribution that displays the probability for a t-value of 2.

The sampling distribution indicates that each of the two shaded regions has a probability of 0.02963—for a total of 0.05926. That’s the p-value! The graph shows that the test statistic falls within these areas almost 6% of the time when the null hypothesis is true in the population.

While this likelihood seems small, it’s not low enough to justify rejecting the null under the standard significance level of 0.05. P-value results are always consistent with the critical value method. Learn more about using test statistics to find p values .

While test statistics are a crucial part of hypothesis testing, you’ll probably let your statistical software calculate the p-value for the test. However, understanding test statistics will boost your comprehension of what a hypothesis test actually assesses.

Reader Interactions

July 5, 2024 at 8:21 am

“As the observed values progressively fail to match the observed values, the numerator increases, causing the test statistic to rise from zero”.

Sir, this sentence is written in the Chi-squared Test heading. There the observed value is written twice. I think the second one to be replaced with ‘expected values’.

July 5, 2024 at 4:10 pm

Thanks so much, Dr. Raj. You’re correct about the typo and I’ve made the correction.

May 9, 2024 at 1:40 am

Thank you very much (great page on one and two-tailed tests)!

May 6, 2024 at 12:17 pm

I would like to ask a question. If only positive numbers are the possible values in a sample (e.g. absolute values without 0), is it meaningful to test if the sample is significantly different from zero (using for example a one sample t-test or a Wilcoxon signed-rank test) or can I assume that if given a large enough sample, the result will by definition be significant (even if a small or very variable sample results in a non-significant hypothesis test).

Thank you very much,

May 6, 2024 at 4:35 pm

If you’re talking about the raw values you’re assessing using a one-sample t-test, it doesn’t make sense to compare them to zero given your description of the data. You know that the mean can’t possibly equal zero. The mean must be some positive value. Yes, in this scenario, if you have a large enough sample size, you should get statistically significant results. So, that t-test isn’t tell you anything that you don’t already know!

However, you should be aware of several things. The 1-sample test can compare your sample mean to values other than zero. Typically, you’ll need to specify the value of the null hypothesis for your software. This value is the comparison value. The test determines whether your sample data provide enough evidence to conclude that the population mean does not equal the null hypothesis value you specify. You’ll need to specify the value because there is no obvious default value to use. Every 1-sample t-test has its subject-area context with a value that makes sense for its null hypothesis value and it is frequently not zero.

I suspect that you’re getting tripped up with the fact that t-tests use a t-value of zero for its null hypothesis value. That doesn’t mean your 1-sample t-test is comparing your sample mean to zero. The test converts your data to a single t-value and compares the t-value to zero. But your actual null hypothesis value can be something else. It’s just converting your sample to a standardized value to use for testing. So, while the t-test compares your sample’s t-value to zero, you can actually compare your sample mean to any value you specify. You need to use a value that makes sense for your subject area.

I hope that makes sense!

May 8, 2024 at 8:37 am

Thank you very much Jim, this helps a lot! Actually, the value I would like to compare my sample to is zero, but I just couldn’t find the right way to test it apparently (it’s about EEG data). The original data was a sample of numbers between -1 and +1, with the question if they are significantly different from zero in either direction (in which case a one sample t-test makes sense I guess, since the sample mean can in fact be zero). However, since a sample mean of 0 can also occur if half of the sample differs in the negative, and the other half in the positive direction, I also wanted to test if there is a divergence from 0 in ‘absolute’ terms – that’s how the absolute valued numbers came about (I know that absolute values can also be zero, but in this specific case, they were all positive numbers) And a special thanks for the last paragraph – I will definitely keep in mind, it is a potential point of confusion.

May 8, 2024 at 8:33 pm

You can use a 1-sample t test for both cases but you’ll need to set them up slightly different. To detect a positive or negative difference from zero, use a 2-tailed test. For the case with absolute values, use a one-tailed test with a critical region in the positive end. To learn more, read about One- and Two-Tailed Tests Explained . Use zero for the comparison value in both cases.

February 12, 2024 at 1:00 am

Very helpful and well articulated! Thanks Jim 🙂

September 18, 2023 at 10:01 am

Thank you for brief explanation.

July 25, 2022 at 8:32 am

the content was helpful to me. thank you

Comments and Questions Cancel reply

What is The Null Hypothesis & When Do You Reject The Null Hypothesis

Julia Simkus

Editor at Simply Psychology

BA (Hons) Psychology, Princeton University

Julia Simkus is a graduate of Princeton University with a Bachelor of Arts in Psychology. She is currently studying for a Master's Degree in Counseling for Mental Health and Wellness in September 2023. Julia's research has been published in peer reviewed journals.

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Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Associate Editor for Simply Psychology

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On This Page:

A null hypothesis is a statistical concept suggesting no significant difference or relationship between measured variables. It’s the default assumption unless empirical evidence proves otherwise.

The null hypothesis states no relationship exists between the two variables being studied (i.e., one variable does not affect the other).

The null hypothesis is the statement that a researcher or an investigator wants to disprove.

Testing the null hypothesis can tell you whether your results are due to the effects of manipulating the dependent variable or due to random chance.

How to Write a Null Hypothesis

Null hypotheses (H0) start as research questions that the investigator rephrases as statements indicating no effect or relationship between the independent and dependent variables.

It is a default position that your research aims to challenge or confirm.

For example, if studying the impact of exercise on weight loss, your null hypothesis might be:

There is no significant difference in weight loss between individuals who exercise daily and those who do not.

Examples of Null Hypotheses

Research Question	Null Hypothesis
Do teenagers use cell phones more than adults?	Teenagers and adults use cell phones the same amount.
Do tomato plants exhibit a higher rate of growth when planted in compost rather than in soil?	Tomato plants show no difference in growth rates when planted in compost rather than soil.
Does daily meditation decrease the incidence of depression?	Daily meditation does not decrease the incidence of depression.
Does daily exercise increase test performance?	There is no relationship between daily exercise time and test performance.
Does the new vaccine prevent infections?	The vaccine does not affect the infection rate.
Does flossing your teeth affect the number of cavities?	Flossing your teeth has no effect on the number of cavities.

When Do We Reject The Null Hypothesis?

We reject the null hypothesis when the data provide strong enough evidence to conclude that it is likely incorrect. This often occurs when the p-value (probability of observing the data given the null hypothesis is true) is below a predetermined significance level.

If the collected data does not meet the expectation of the null hypothesis, a researcher can conclude that the data lacks sufficient evidence to back up the null hypothesis, and thus the null hypothesis is rejected.

Rejecting the null hypothesis means that a relationship does exist between a set of variables and the effect is statistically significant ( p > 0.05).

If the data collected from the random sample is not statistically significance , then the null hypothesis will be accepted, and the researchers can conclude that there is no relationship between the variables.

You need to perform a statistical test on your data in order to evaluate how consistent it is with the null hypothesis. A p-value is one statistical measurement used to validate a hypothesis against observed data.

Calculating the p-value is a critical part of null-hypothesis significance testing because it quantifies how strongly the sample data contradicts the null hypothesis.

The level of statistical significance is often expressed as a p -value between 0 and 1. The smaller the p-value, the stronger the evidence that you should reject the null hypothesis.

Probability and statistical significance in ab testing. Statistical significance in a b experiments

Usually, a researcher uses a confidence level of 95% or 99% (p-value of 0.05 or 0.01) as general guidelines to decide if you should reject or keep the null.

When your p-value is less than or equal to your significance level, you reject the null hypothesis.

In other words, smaller p-values are taken as stronger evidence against the null hypothesis. Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis.

In this case, the sample data provides insufficient data to conclude that the effect exists in the population.

Because you can never know with complete certainty whether there is an effect in the population, your inferences about a population will sometimes be incorrect.

When you incorrectly reject the null hypothesis, it’s called a type I error. When you incorrectly fail to reject it, it’s called a type II error.

Why Do We Never Accept The Null Hypothesis?

The reason we do not say “accept the null” is because we are always assuming the null hypothesis is true and then conducting a study to see if there is evidence against it. And, even if we don’t find evidence against it, a null hypothesis is not accepted.

A lack of evidence only means that you haven’t proven that something exists. It does not prove that something doesn’t exist.

It is risky to conclude that the null hypothesis is true merely because we did not find evidence to reject it. It is always possible that researchers elsewhere have disproved the null hypothesis, so we cannot accept it as true, but instead, we state that we failed to reject the null.

One can either reject the null hypothesis, or fail to reject it, but can never accept it.

Why Do We Use The Null Hypothesis?

We can never prove with 100% certainty that a hypothesis is true; We can only collect evidence that supports a theory. However, testing a hypothesis can set the stage for rejecting or accepting this hypothesis within a certain confidence level.

The null hypothesis is useful because it can tell us whether the results of our study are due to random chance or the manipulation of a variable (with a certain level of confidence).

A null hypothesis is rejected if the measured data is significantly unlikely to have occurred and a null hypothesis is accepted if the observed outcome is consistent with the position held by the null hypothesis.

Rejecting the null hypothesis sets the stage for further experimentation to see if a relationship between two variables exists.

Hypothesis testing is a critical part of the scientific method as it helps decide whether the results of a research study support a particular theory about a given population. Hypothesis testing is a systematic way of backing up researchers’ predictions with statistical analysis.

It helps provide sufficient statistical evidence that either favors or rejects a certain hypothesis about the population parameter.

Purpose of a Null Hypothesis

The primary purpose of the null hypothesis is to disprove an assumption.
Whether rejected or accepted, the null hypothesis can help further progress a theory in many scientific cases.
A null hypothesis can be used to ascertain how consistent the outcomes of multiple studies are.

Do you always need both a Null Hypothesis and an Alternative Hypothesis?

The null (H0) and alternative (Ha or H1) hypotheses are two competing claims that describe the effect of the independent variable on the dependent variable. They are mutually exclusive, which means that only one of the two hypotheses can be true.

While the null hypothesis states that there is no effect in the population, an alternative hypothesis states that there is statistical significance between two variables.

The goal of hypothesis testing is to make inferences about a population based on a sample. In order to undertake hypothesis testing, you must express your research hypothesis as a null and alternative hypothesis. Both hypotheses are required to cover every possible outcome of the study.

What is the difference between a null hypothesis and an alternative hypothesis?

The alternative hypothesis is the complement to the null hypothesis. The null hypothesis states that there is no effect or no relationship between variables, while the alternative hypothesis claims that there is an effect or relationship in the population.

It is the claim that you expect or hope will be true. The null hypothesis and the alternative hypothesis are always mutually exclusive, meaning that only one can be true at a time.

What are some problems with the null hypothesis?

One major problem with the null hypothesis is that researchers typically will assume that accepting the null is a failure of the experiment. However, accepting or rejecting any hypothesis is a positive result. Even if the null is not refuted, the researchers will still learn something new.

Why can a null hypothesis not be accepted?

We can either reject or fail to reject a null hypothesis, but never accept it. If your test fails to detect an effect, this is not proof that the effect doesn’t exist. It just means that your sample did not have enough evidence to conclude that it exists.

We can’t accept a null hypothesis because a lack of evidence does not prove something that does not exist. Instead, we fail to reject it.

Failing to reject the null indicates that the sample did not provide sufficient enough evidence to conclude that an effect exists.

If the p-value is greater than the significance level, then you fail to reject the null hypothesis.

Is a null hypothesis directional or non-directional?

A hypothesis test can either contain an alternative directional hypothesis or a non-directional alternative hypothesis. A directional hypothesis is one that contains the less than (“<“) or greater than (“>”) sign.

A nondirectional hypothesis contains the not equal sign (“≠”). However, a null hypothesis is neither directional nor non-directional.

A null hypothesis is a prediction that there will be no change, relationship, or difference between two variables.

The directional hypothesis or nondirectional hypothesis would then be considered alternative hypotheses to the null hypothesis.

Gill, J. (1999). The insignificance of null hypothesis significance testing. Political research quarterly , 52 (3), 647-674.

Krueger, J. (2001). Null hypothesis significance testing: On the survival of a flawed method. American Psychologist , 56 (1), 16.

Masson, M. E. (2011). A tutorial on a practical Bayesian alternative to null-hypothesis significance testing. Behavior research methods , 43 , 679-690.

Nickerson, R. S. (2000). Null hypothesis significance testing: a review of an old and continuing controversy. Psychological methods , 5 (2), 241.

Rozeboom, W. W. (1960). The fallacy of the null-hypothesis significance test. Psychological bulletin , 57 (5), 416.

9.1 Null and Alternative Hypotheses

The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 , the — null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.

H a —, the alternative hypothesis: a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are reject H 0 if the sample information favors the alternative hypothesis or do not reject H 0 or decline to reject H 0 if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in H 0 and H a :


equal (=)	not equal (≠) greater than (>) less than (<)
greater than or equal to (≥)	less than (<)
less than or equal to (≤)	more than (>)

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example 9.1

H 0 : No more than 30 percent of the registered voters in Santa Clara County voted in the primary election. p ≤ 30 H a : More than 30 percent of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25 percent. State the null and alternative hypotheses.

Example 9.2

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are the following: H 0 : μ = 2.0 H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

H 0 : μ __ 66
H a : μ __ 66

Example 9.3

We want to test if college students take fewer than five years to graduate from college, on the average. The null and alternative hypotheses are the following: H 0 : μ ≥ 5 H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

H 0 : μ __ 45
H a : μ __ 45

Example 9.4

An article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third of the students pass. The same article stated that 6.6 percent of U.S. students take advanced placement exams and 4.4 percent pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6 percent. State the null and alternative hypotheses. H 0 : p ≤ 0.066 H a : p > 0.066

On a state driver’s test, about 40 percent pass the test on the first try. We want to test if more than 40 percent pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

H 0 : p __ 0.40
H a : p __ 0.40

Collaborative Exercise

Bring to class a newspaper, some news magazines, and some internet articles. In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

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Knowledge Base
Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

Null hypothesis (H 0 ): There’s no effect in the population .
Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample.

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

	( )

Does tooth flossing affect the number of cavities?	Tooth flossing has on the number of cavities.	test: The mean number of cavities per person does not differ between the flossing group (µ ) and the non-flossing group (µ ) in the population; µ = µ .
Does the amount of text highlighted in the textbook affect exam scores?	The amount of text highlighted in the textbook has on exam scores.	: There is no relationship between the amount of text highlighted and exam scores in the population; β = 0.
Does daily meditation decrease the incidence of depression?	Daily meditation the incidence of depression.*	test: The proportion of people with depression in the daily-meditation group ( ) is greater than or equal to the no-meditation group ( ) in the population; ≥ .

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.



Does tooth flossing affect the number of cavities?	Tooth flossing has an on the number of cavities.	test: The mean number of cavities per person differs between the flossing group (µ ) and the non-flossing group (µ ) in the population; µ ≠ µ .
Does the amount of text highlighted in a textbook affect exam scores?	The amount of text highlighted in the textbook has an on exam scores.	: There is a relationship between the amount of text highlighted and exam scores in the population; β ≠ 0.
Does daily meditation decrease the incidence of depression?	Daily meditation the incidence of depression.	test: The proportion of people with depression in the daily-meditation group ( ) is less than the no-meditation group ( ) in the population; < .

Null and alternative hypotheses are similar in some ways:

They’re both answers to the research question
They both make claims about the population
They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.


	A claim that there is in the population.	A claim that there is in the population.


	Equality symbol (=, ≥, or ≤)	Inequality symbol (≠, <, or >)
	Rejected	Supported
	Failed to reject	Not supported

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

	( )
test with two groups	The mean dependent variable does not differ between group 1 (µ ) and group 2 (µ ) in the population; µ = µ .	The mean dependent variable differs between group 1 (µ ) and group 2 (µ ) in the population; µ ≠ µ .
with three groups	The mean dependent variable does not differ between group 1 (µ ), group 2 (µ ), and group 3 (µ ) in the population; µ = µ = µ .	The mean dependent variable of group 1 (µ ), group 2 (µ ), and group 3 (µ ) are not all equal in the population.
	There is no correlation between independent variable and dependent variable in the population; ρ = 0.	There is a correlation between independent variable and dependent variable in the population; ρ ≠ 0.
	There is no relationship between independent variable and dependent variable in the population; β = 0.	There is a relationship between independent variable and dependent variable in the population; β ≠ 0.
Two-proportions test	The dependent variable expressed as a proportion does not differ between group 1 ( ) and group 2 ( ) in the population; = .	The dependent variable expressed as a proportion differs between group 1 ( ) and group 2 ( ) in the population; ≠ .

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Chapter 13: Inferential Statistics

Understanding Null Hypothesis Testing

Learning Objectives

Explain the purpose of null hypothesis testing, including the role of sampling error.
Describe the basic logic of null hypothesis testing.
Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables for a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 clinically depressed adults and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for clinically depressed adults).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of clinically depressed adults, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called sampling error . (Note that the term error here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s r value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

There is a relationship in the population, and the relationship in the sample reflects this.
There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the null hypothesis (often symbolized H 0 and read as “H-naught”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the alternative hypothesis (often symbolized as H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
Determine how likely the sample relationship would be if the null hypothesis were true.
If the sample relationship would be extremely unlikely, then reject the null hypothesis in favour of the alternative hypothesis. If it would not be extremely unlikely, then retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of d = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favour of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the p value . A low p value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high p value means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the p value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called α (alpha) and is almost always set to .05. If there is less than a 5% chance of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to conclude that it is true. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood p Value

The p value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [1] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the p value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the p value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The p value is really the probability of a result at least as extreme as the sample result if the null hypothesis were true. So a p value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the p value is not the probability that any particular hypothesis is true or false. Instead, it is the probability of obtaining the sample result if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the p value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the p value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s d is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s d is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word Yes , then this combination would be statistically significant for both Cohen’s d and Pearson’s r . If it contains the word No , then it would not be statistically significant for either. There is one cell where the decision for d and r would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Table 13.1 How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant
Sample Size	Weak relationship	Medium-strength relationship	Strong relationship
Small ( = 20)	No	No	= Maybe = Yes
Medium ( = 50)	No	Yes	Yes
Large ( = 100)	= Yes = No	Yes	Yes
Extra large ( = 500)	Yes	Yes	Yes

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [2] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word significant can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the statistical significance of a result and the practical significance of that result. Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Key Takeaways

Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favour of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
The probability of obtaining the sample result if the null hypothesis were true (the p value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.
The correlation between two variables is r = −.78 based on a sample size of 137.
The mean score on a psychological characteristic for women is 25 ( SD = 5) and the mean score for men is 24 ( SD = 5). There were 12 women and 10 men in this study.
In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
A student finds a correlation of r = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.

Long Descriptions

“Null Hypothesis” long description: A comic depicting a man and a woman talking in the foreground. In the background is a child working at a desk. The man says to the woman, “I can’t believe schools are still teaching kids about the null hypothesis. I remember reading a big study that conclusively disproved it years ago.” [Return to “Null Hypothesis”]

“Conditional Risk” long description: A comic depicting two hikers beside a tree during a thunderstorm. A bolt of lightning goes “crack” in the dark sky as thunder booms. One of the hikers says, “Whoa! We should get inside!” The other hiker says, “It’s okay! Lightning only kills about 45 Americans a year, so the chances of dying are only one in 7,000,000. Let’s go on!” The comic’s caption says, “The annual death rate among people who know that statistic is one in six.” [Return to “Conditional Risk”]

Media Attributions

Null Hypothesis by XKCD CC BY-NC (Attribution NonCommercial)
Conditional Risk by XKCD CC BY-NC (Attribution NonCommercial)
Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

Values in a population that correspond to variables measured in a study.

The random variability in a statistic from sample to sample.

A formal approach to deciding between two interpretations of a statistical relationship in a sample.

The idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error.

The idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

When the relationship found in the sample would be extremely unlikely, the idea that the relationship occurred “by chance” is rejected.

When the relationship found in the sample is likely to have occurred by chance, the null hypothesis is not rejected.

The probability that, if the null hypothesis were true, the result found in the sample would occur.

How low the p value must be before the sample result is considered unlikely in null hypothesis testing.

When there is less than a 5% chance of a result as extreme as the sample result occurring and the null hypothesis is rejected.

Research Methods in Psychology - 2nd Canadian Edition Copyright © 2015 by Paul C. Price, Rajiv Jhangiani, & I-Chant A. Chiang is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Null Hypothesis Definition and Examples, How to State

What is the null hypothesis, how to state the null hypothesis, null hypothesis overview.

Why is it Called the “Null”?

The word “null” in this context means that it’s a commonly accepted fact that researchers work to nullify . It doesn’t mean that the statement is null (i.e. amounts to nothing) itself! (Perhaps the term should be called the “nullifiable hypothesis” as that might cause less confusion).

Why Do I need to Test it? Why not just prove an alternate one?

The short answer is, as a scientist, you are required to ; It’s part of the scientific process. Science uses a battery of processes to prove or disprove theories, making sure than any new hypothesis has no flaws. Including both a null and an alternate hypothesis is one safeguard to ensure your research isn’t flawed. Not including the null hypothesis in your research is considered very bad practice by the scientific community. If you set out to prove an alternate hypothesis without considering it, you are likely setting yourself up for failure. At a minimum, your experiment will likely not be taken seriously.

Null hypothesis : H 0 : The world is flat.
Alternate hypothesis: The world is round.

Several scientists, including Copernicus , set out to disprove the null hypothesis. This eventually led to the rejection of the null and the acceptance of the alternate. Most people accepted it — the ones that didn’t created the Flat Earth Society !. What would have happened if Copernicus had not disproved the it and merely proved the alternate? No one would have listened to him. In order to change people’s thinking, he first had to prove that their thinking was wrong .

How to State the Null Hypothesis from a Word Problem

You’ll be asked to convert a word problem into a hypothesis statement in statistics that will include a null hypothesis and an alternate hypothesis . Breaking your problem into a few small steps makes these problems much easier to handle.

Step 2: Convert the hypothesis to math . Remember that the average is sometimes written as μ.

H 1 : μ > 8.2

Broken down into (somewhat) English, that’s H 1 (The hypothesis): μ (the average) > (is greater than) 8.2

Step 3: State what will happen if the hypothesis doesn’t come true. If the recovery time isn’t greater than 8.2 weeks, there are only two possibilities, that the recovery time is equal to 8.2 weeks or less than 8.2 weeks.

H 0 : μ ≤ 8.2

Broken down again into English, that’s H 0 (The null hypothesis): μ (the average) ≤ (is less than or equal to) 8.2

How to State the Null Hypothesis: Part Two

But what if the researcher doesn’t have any idea what will happen.

Example Problem: A researcher is studying the effects of radical exercise program on knee surgery patients. There is a good chance the therapy will improve recovery time, but there’s also the possibility it will make it worse. Average recovery times for knee surgery patients is 8.2 weeks.

Step 1: State what will happen if the experiment doesn’t make any difference. That’s the null hypothesis–that nothing will happen. In this experiment, if nothing happens, then the recovery time will stay at 8.2 weeks.

H 0 : μ = 8.2

Broken down into English, that’s H 0 (The null hypothesis): μ (the average) = (is equal to) 8.2

Step 2: Figure out the alternate hypothesis . The alternate hypothesis is the opposite of the null hypothesis. In other words, what happens if our experiment makes a difference?

H 1 : μ ≠ 8.2

In English again, that’s H 1 (The alternate hypothesis): μ (the average) ≠ (is not equal to) 8.2

That’s How to State the Null Hypothesis!

Check out our Youtube channel for more stats tips!

Gonick, L. (1993). The Cartoon Guide to Statistics . HarperPerennial. Kotz, S.; et al., eds. (2006), Encyclopedia of Statistical Sciences , Wiley.

13.1 Understanding Null Hypothesis Testing

Learning objectives.

Explain the purpose of null hypothesis testing, including the role of sampling error.
Describe the basic logic of null hypothesis testing.
Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables in a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 adults with clinical depression and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for adults with clinical depression).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of adults with clinical depression, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called sampling error . (Note that the term error here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

In fact, any statistical relationship in a sample can be interpreted in two ways:

There is a relationship in the population, and the relationship in the sample reflects this.
There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
Determine how likely the sample relationship would be if the null hypothesis were true.
If the sample relationship would be extremely unlikely, then reject the null hypothesis in favor of the alternative hypothesis. If it would not be extremely unlikely, then retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of d = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favor of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the p value . A low p value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A p value that is not low means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the p value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called α (alpha) and is almost always set to .05. If there is a 5% chance or less of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to reject it. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood p Value

“Null Hypothesis” retrieved from http://imgs.xkcd.com/comics/null_hypothesis.png (CC-BY-NC 2.5)

Role of Sample Size and Relationship Strength



Sample Size	Weak	Medium	Strong
Small ( = 20)	No	No	= Maybe = Yes
Medium ( = 50)	No	Yes	Yes
Large ( = 100)	= Yes = No	Yes	Yes
Extra large ( = 500)	Yes	Yes	Yes

Statistical Significance Versus Practical Significance

“Conditional Risk” retrieved from http://imgs.xkcd.com/comics/conditional_risk.png (CC-BY-NC 2.5)

Key Takeaways

Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favor of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
The probability of obtaining the sample result if the null hypothesis were true (the p value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.
The correlation between two variables is r = −.78 based on a sample size of 137.
The mean score on a psychological characteristic for women is 25 ( SD = 5) and the mean score for men is 24 ( SD = 5). There were 12 women and 10 men in this study.
In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
A student finds a correlation of r = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.
Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

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In statistical analysis, the null hypothesis assumes there is no meaningful relationship between two variables. Testing the null hypothesis can tell you whether your results are due to the effect of manipulating a dependent variable or due to chance. It's often used in conjunction with an alternative hypothesis, which assumes there is, in fact, a relationship between two variables.

The null hypothesis is among the easiest hypothesis to test using statistical analysis, making it perhaps the most valuable hypothesis for the scientific method. By evaluating a null hypothesis in addition to another hypothesis, researchers can support their conclusions with a higher level of confidence. Below are examples of how you might formulate a null hypothesis to fit certain questions.

What Is the Null Hypothesis?

The null hypothesis states there is no relationship between the measured phenomenon (the dependent variable ) and the independent variable , which is the variable an experimenter typically controls or changes. You do not need to believe that the null hypothesis is true to test it. On the contrary, you will likely suspect there is a relationship between a set of variables. One way to prove that this is the case is to reject the null hypothesis. Rejecting a hypothesis does not mean an experiment was "bad" or that it didn't produce results. In fact, it is often one of the first steps toward further inquiry.

To distinguish it from other hypotheses , the null hypothesis is written as H 0 (which is read as “H-nought,” "H-null," or "H-zero"). A significance test is used to determine the likelihood that the results supporting the null hypothesis are not due to chance. A confidence level of 95% or 99% is common. Keep in mind, even if the confidence level is high, there is still a small chance the null hypothesis is not true, perhaps because the experimenter did not account for a critical factor or because of chance. This is one reason why it's important to repeat experiments.

Examples of the Null Hypothesis

To write a null hypothesis, first start by asking a question. Rephrase that question in a form that assumes no relationship between the variables. In other words, assume a treatment has no effect. Write your hypothesis in a way that reflects this.


Are teens better at math than adults?	Age has no effect on mathematical ability.
Does taking aspirin every day reduce the chance of having a heart attack?	Taking aspirin daily does not affect heart attack risk.
Do teens use cell phones to access the internet more than adults?	Age has no effect on how cell phones are used for internet access.
Do cats care about the color of their food?	Cats express no food preference based on color.
Does chewing willow bark relieve pain?	There is no difference in pain relief after chewing willow bark versus taking a placebo.

Other Types of Hypotheses

In addition to the null hypothesis, the alternative hypothesis is also a staple in traditional significance tests . It's essentially the opposite of the null hypothesis because it assumes the claim in question is true. For the first item in the table above, for example, an alternative hypothesis might be "Age does have an effect on mathematical ability."

Key Takeaways

In hypothesis testing, the null hypothesis assumes no relationship between two variables, providing a baseline for statistical analysis.
Rejecting the null hypothesis suggests there is evidence of a relationship between variables.
By formulating a null hypothesis, researchers can systematically test assumptions and draw more reliable conclusions from their experiments.
What Are Examples of a Hypothesis?
Random Error vs. Systematic Error
Six Steps of the Scientific Method
What Is a Hypothesis? (Science)
Scientific Method Flow Chart
What Are the Elements of a Good Hypothesis?
Scientific Method Vocabulary Terms
Understanding Simple vs Controlled Experiments
The Role of a Controlled Variable in an Experiment
What Is an Experimental Constant?
What Is a Testable Hypothesis?
Scientific Hypothesis Examples
What Is the Difference Between a Control Variable and Control Group?
DRY MIX Experiment Variables Acronym
What Is a Controlled Experiment?
Scientific Variable

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Keyboard Shortcuts

1.2 - the 7 step process of statistical hypothesis testing.

We will cover the seven steps one by one.

Step 1: State the Null Hypothesis

The null hypothesis can be thought of as the opposite of the "guess" the researchers made. In the example presented in the previous section, the biologist "guesses" plant height will be different for the various fertilizers. So the null hypothesis would be that there will be no difference among the groups of plants. Specifically, in more statistical language the null for an ANOVA is that the means are the same. We state the null hypothesis as:

\(H_0 \colon \mu_1 = \mu_2 = ⋯ = \mu_T\)

for T levels of an experimental treatment.

Step 2: State the Alternative Hypothesis

\(H_A \colon \text{ treatment level means not all equal}\)

The alternative hypothesis is stated in this way so that if the null is rejected, there are many alternative possibilities.

For example, \(\mu_1\ne \mu_2 = ⋯ = \mu_T\) is one possibility, as is \(\mu_1=\mu_2\ne\mu_3= ⋯ =\mu_T\). Many people make the mistake of stating the alternative hypothesis as \(\mu_1\ne\mu_2\ne⋯\ne\mu_T\) which says that every mean differs from every other mean. This is a possibility, but only one of many possibilities. A simple way of thinking about this is that at least one mean is different from all others. To cover all alternative outcomes, we resort to a verbal statement of "not all equal" and then follow up with mean comparisons to find out where differences among means exist. In our example, a possible outcome would be that fertilizer 1 results in plants that are exceptionally tall, but fertilizers 2, 3, and the control group may not differ from one another.

Step 3: Set \(\alpha\)

If we look at what can happen in a hypothesis test, we can construct the following contingency table:

Decision	In Reality
Decision	\(H_0\) is TRUE	\(H_0\) is FALSE
Accept \(H_0\)	correct	Type II Error \(\beta\) = probability of Type II Error
Reject \(H_0\)	Type I Error \(\alpha\) = probability of Type I Error	correct

You should be familiar with Type I and Type II errors from your introductory courses. It is important to note that we want to set \(\alpha\) before the experiment ( a-priori ) because the Type I error is the more grievous error to make. The typical value of \(\alpha\) is 0.05, establishing a 95% confidence level. For this course, we will assume \(\alpha\) =0.05, unless stated otherwise.

Step 4: Collect Data

Remember the importance of recognizing whether data is collected through an experimental design or observational study.

Step 5: Calculate a test statistic

For categorical treatment level means, we use an F- statistic, named after R.A. Fisher. We will explore the mechanics of computing the F- statistic beginning in Lesson 2. The F- value we get from the data is labeled \(F_{\text{calculated}}\).

Step 6: Construct Acceptance / Rejection regions

As with all other test statistics, a threshold (critical) value of F is established. This F- value can be obtained from statistical tables or software and is referred to as \(F_{\text{critical}}\) or \(F_\alpha\). As a reminder, this critical value is the minimum value of the test statistic (in this case \(F_{\text{calculated}}\)) for us to reject the null.

The F- distribution, \(F_\alpha\), and the location of acceptance/rejection regions are shown in the graph below:

Step 7: Based on Steps 5 and 6, draw a conclusion about \(H_0\)

If \(F_{\text{calculated}}\) is larger than \(F_\alpha\), then you are in the rejection region and you can reject the null hypothesis with \(\left(1-\alpha \right)\) level of confidence.

Note that modern statistical software condenses Steps 6 and 7 by providing a p -value. The p -value here is the probability of getting an \(F_{\text{calculated}}\) even greater than what you observe assuming the null hypothesis is true. If by chance, the \(F_{\text{calculated}} = F_\alpha\), then the p -value would be exactly equal to \(\alpha\). With larger \(F_{\text{calculated}}\) values, we move further into the rejection region and the p- value becomes less than \(\alpha\). So, the decision rule is as follows:

If the p- value obtained from the ANOVA is less than \(\alpha\), then reject \(H_0\) in favor of \(H_A\).

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PMC5635437.1 ; 2015 Aug 25
PMC5635437.2 ; 2016 Jul 13
➤ PMC5635437.3; 2016 Oct 10

Null hypothesis significance testing: a short tutorial

Cyril pernet.

1 Centre for Clinical Brain Sciences (CCBS), Neuroimaging Sciences, The University of Edinburgh, Edinburgh, UK

Version Changes

Revised. amendments from version 2.

This v3 includes minor changes that reflect the 3rd reviewers' comments - in particular the theoretical vs. practical difference between Fisher and Neyman-Pearson. Additional information and reference is also included regarding the interpretation of p-value for low powered studies.

Peer Review Summary

Review date	Reviewer name(s)	Version reviewed	Review status
	Dorothy Vera Margaret Bishop		Approved with Reservations
	Stephen J. Senn		Approved
	Stephen J. Senn		Approved with Reservations
	Marcel ALM van Assen		Not Approved
	Daniel Lakens		Not Approved

Although thoroughly criticized, null hypothesis significance testing (NHST) remains the statistical method of choice used to provide evidence for an effect, in biological, biomedical and social sciences. In this short tutorial, I first summarize the concepts behind the method, distinguishing test of significance (Fisher) and test of acceptance (Newman-Pearson) and point to common interpretation errors regarding the p-value. I then present the related concepts of confidence intervals and again point to common interpretation errors. Finally, I discuss what should be reported in which context. The goal is to clarify concepts to avoid interpretation errors and propose reporting practices.

The Null Hypothesis Significance Testing framework

NHST is a method of statistical inference by which an experimental factor is tested against a hypothesis of no effect or no relationship based on a given observation. The method is a combination of the concepts of significance testing developed by Fisher in 1925 and of acceptance based on critical rejection regions developed by Neyman & Pearson in 1928 . In the following I am first presenting each approach, highlighting the key differences and common misconceptions that result from their combination into the NHST framework (for a more mathematical comparison, along with the Bayesian method, see Christensen, 2005 ). I next present the related concept of confidence intervals. I finish by discussing practical aspects in using NHST and reporting practice.

Fisher, significance testing, and the p-value

The method developed by ( Fisher, 1934 ; Fisher, 1955 ; Fisher, 1959 ) allows to compute the probability of observing a result at least as extreme as a test statistic (e.g. t value), assuming the null hypothesis of no effect is true. This probability or p-value reflects (1) the conditional probability of achieving the observed outcome or larger: p(Obs≥t|H0), and (2) is therefore a cumulative probability rather than a point estimate. It is equal to the area under the null probability distribution curve from the observed test statistic to the tail of the null distribution ( Turkheimer et al. , 2004 ). The approach proposed is of ‘proof by contradiction’ ( Christensen, 2005 ), we pose the null model and test if data conform to it.

In practice, it is recommended to set a level of significance (a theoretical p-value) that acts as a reference point to identify significant results, that is to identify results that differ from the null-hypothesis of no effect. Fisher recommended using p=0.05 to judge whether an effect is significant or not as it is roughly two standard deviations away from the mean for the normal distribution ( Fisher, 1934 page 45: ‘The value for which p=.05, or 1 in 20, is 1.96 or nearly 2; it is convenient to take this point as a limit in judging whether a deviation is to be considered significant or not’). A key aspect of Fishers’ theory is that only the null-hypothesis is tested, and therefore p-values are meant to be used in a graded manner to decide whether the evidence is worth additional investigation and/or replication ( Fisher, 1971 page 13: ‘it is open to the experimenter to be more or less exacting in respect of the smallness of the probability he would require […]’ and ‘no isolated experiment, however significant in itself, can suffice for the experimental demonstration of any natural phenomenon’). How small the level of significance is, is thus left to researchers.

What is not a p-value? Common mistakes

The p-value is not an indication of the strength or magnitude of an effect . Any interpretation of the p-value in relation to the effect under study (strength, reliability, probability) is wrong, since p-values are conditioned on H0. In addition, while p-values are randomly distributed (if all the assumptions of the test are met) when there is no effect, their distribution depends of both the population effect size and the number of participants, making impossible to infer strength of effect from them.

Similarly, 1-p is not the probability to replicate an effect . Often, a small value of p is considered to mean a strong likelihood of getting the same results on another try, but again this cannot be obtained because the p-value is not informative on the effect itself ( Miller, 2009 ). Because the p-value depends on the number of subjects, it can only be used in high powered studies to interpret results. In low powered studies (typically small number of subjects), the p-value has a large variance across repeated samples, making it unreliable to estimate replication ( Halsey et al. , 2015 ).

A (small) p-value is not an indication favouring a given hypothesis . Because a low p-value only indicates a misfit of the null hypothesis to the data, it cannot be taken as evidence in favour of a specific alternative hypothesis more than any other possible alternatives such as measurement error and selection bias ( Gelman, 2013 ). Some authors have even argued that the more (a priori) implausible the alternative hypothesis, the greater the chance that a finding is a false alarm ( Krzywinski & Altman, 2013 ; Nuzzo, 2014 ).

The p-value is not the probability of the null hypothesis p(H0), of being true, ( Krzywinski & Altman, 2013 ). This common misconception arises from a confusion between the probability of an observation given the null p(Obs≥t|H0) and the probability of the null given an observation p(H0|Obs≥t) that is then taken as an indication for p(H0) (see Nickerson, 2000 ).

Neyman-Pearson, hypothesis testing, and the α-value

Neyman & Pearson (1933) proposed a framework of statistical inference for applied decision making and quality control. In such framework, two hypotheses are proposed: the null hypothesis of no effect and the alternative hypothesis of an effect, along with a control of the long run probabilities of making errors. The first key concept in this approach, is the establishment of an alternative hypothesis along with an a priori effect size. This differs markedly from Fisher who proposed a general approach for scientific inference conditioned on the null hypothesis only. The second key concept is the control of error rates . Neyman & Pearson (1928) introduced the notion of critical intervals, therefore dichotomizing the space of possible observations into correct vs. incorrect zones. This dichotomization allows distinguishing correct results (rejecting H0 when there is an effect and not rejecting H0 when there is no effect) from errors (rejecting H0 when there is no effect, the type I error, and not rejecting H0 when there is an effect, the type II error). In this context, alpha is the probability of committing a Type I error in the long run. Alternatively, Beta is the probability of committing a Type II error in the long run.

The (theoretical) difference in terms of hypothesis testing between Fisher and Neyman-Pearson is illustrated on Figure 1 . In the 1 st case, we choose a level of significance for observed data of 5%, and compute the p-value. If the p-value is below the level of significance, it is used to reject H0. In the 2 nd case, we set a critical interval based on the a priori effect size and error rates. If an observed statistic value is below and above the critical values (the bounds of the confidence region), it is deemed significantly different from H0. In the NHST framework, the level of significance is (in practice) assimilated to the alpha level, which appears as a simple decision rule: if the p-value is less or equal to alpha, the null is rejected. It is however a common mistake to assimilate these two concepts. The level of significance set for a given sample is not the same as the frequency of acceptance alpha found on repeated sampling because alpha (a point estimate) is meant to reflect the long run probability whilst the p-value (a cumulative estimate) reflects the current probability ( Fisher, 1955 ; Hubbard & Bayarri, 2003 ).

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The figure was prepared with G-power for a one-sided one-sample t-test, with a sample size of 32 subjects, an effect size of 0.45, and error rates alpha=0.049 and beta=0.80. In Fisher’s procedure, only the nil-hypothesis is posed, and the observed p-value is compared to an a priori level of significance. If the observed p-value is below this level (here p=0.05), one rejects H0. In Neyman-Pearson’s procedure, the null and alternative hypotheses are specified along with an a priori level of acceptance. If the observed statistical value is outside the critical region (here [-∞ +1.69]), one rejects H0.

Acceptance or rejection of H0?

The acceptance level α can also be viewed as the maximum probability that a test statistic falls into the rejection region when the null hypothesis is true ( Johnson, 2013 ). Therefore, one can only reject the null hypothesis if the test statistics falls into the critical region(s), or fail to reject this hypothesis. In the latter case, all we can say is that no significant effect was observed, but one cannot conclude that the null hypothesis is true. This is another common mistake in using NHST: there is a profound difference between accepting the null hypothesis and simply failing to reject it ( Killeen, 2005 ). By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot argue against a theory from a non-significant result (absence of evidence is not evidence of absence). To accept the null hypothesis, tests of equivalence ( Walker & Nowacki, 2011 ) or Bayesian approaches ( Dienes, 2014 ; Kruschke, 2011 ) must be used.

Confidence intervals

Confidence intervals (CI) are builds that fail to cover the true value at a rate of alpha, the Type I error rate ( Morey & Rouder, 2011 ) and therefore indicate if observed values can be rejected by a (two tailed) test with a given alpha. CI have been advocated as alternatives to p-values because (i) they allow judging the statistical significance and (ii) provide estimates of effect size. Assuming the CI (a)symmetry and width are correct (but see Wilcox, 2012 ), they also give some indication about the likelihood that a similar value can be observed in future studies. For future studies of the same sample size, 95% CI give about 83% chance of replication success ( Cumming & Maillardet, 2006 ). If sample sizes however differ between studies, CI do not however warranty any a priori coverage.

Although CI provide more information, they are not less subject to interpretation errors (see Savalei & Dunn, 2015 for a review). The most common mistake is to interpret CI as the probability that a parameter (e.g. the population mean) will fall in that interval X% of the time. The correct interpretation is that, for repeated measurements with the same sample sizes, taken from the same population, X% of times the CI obtained will contain the true parameter value ( Tan & Tan, 2010 ). The alpha value has the same interpretation as testing against H0, i.e. we accept that 1-alpha CI are wrong in alpha percent of the times in the long run. This implies that CI do not allow to make strong statements about the parameter of interest (e.g. the mean difference) or about H1 ( Hoekstra et al. , 2014 ). To make a statement about the probability of a parameter of interest (e.g. the probability of the mean), Bayesian intervals must be used.

The (correct) use of NHST

NHST has always been criticized, and yet is still used every day in scientific reports ( Nickerson, 2000 ). One question to ask oneself is what is the goal of a scientific experiment at hand? If the goal is to establish a discrepancy with the null hypothesis and/or establish a pattern of order, because both requires ruling out equivalence, then NHST is a good tool ( Frick, 1996 ; Walker & Nowacki, 2011 ). If the goal is to test the presence of an effect and/or establish some quantitative values related to an effect, then NHST is not the method of choice since testing is conditioned on H0.

While a Bayesian analysis is suited to estimate that the probability that a hypothesis is correct, like NHST, it does not prove a theory on itself, but adds its plausibility ( Lindley, 2000 ). No matter what testing procedure is used and how strong results are, ( Fisher, 1959 p13) reminds us that ‘ […] no isolated experiment, however significant in itself, can suffice for the experimental demonstration of any natural phenomenon'. Similarly, the recent statement of the American Statistical Association ( Wasserstein & Lazar, 2016 ) makes it clear that conclusions should be based on the researchers understanding of the problem in context, along with all summary data and tests, and that no single value (being p-values, Bayesian factor or else) can be used support or invalidate a theory.

What to report and how?

Considering that quantitative reports will always have more information content than binary (significant or not) reports, we can always argue that raw and/or normalized effect size, confidence intervals, or Bayes factor must be reported. Reporting everything can however hinder the communication of the main result(s), and we should aim at giving only the information needed, at least in the core of a manuscript. Here I propose to adopt optimal reporting in the result section to keep the message clear, but have detailed supplementary material. When the hypothesis is about the presence/absence or order of an effect, and providing that a study has sufficient power, NHST is appropriate and it is sufficient to report in the text the actual p-value since it conveys the information needed to rule out equivalence. When the hypothesis and/or the discussion involve some quantitative value, and because p-values do not inform on the effect, it is essential to report on effect sizes ( Lakens, 2013 ), preferably accompanied with confidence or credible intervals. The reasoning is simply that one cannot predict and/or discuss quantities without accounting for variability. For the reader to understand and fully appreciate the results, nothing else is needed.

Because science progress is obtained by cumulating evidence ( Rosenthal, 1991 ), scientists should also consider the secondary use of the data. With today’s electronic articles, there are no reasons for not including all of derived data: mean, standard deviations, effect size, CI, Bayes factor should always be included as supplementary tables (or even better also share raw data). It is also essential to report the context in which tests were performed – that is to report all of the tests performed (all t, F, p values) because of the increase type one error rate due to selective reporting (multiple comparisons and p-hacking problems - Ioannidis, 2005 ). Providing all of this information allows (i) other researchers to directly and effectively compare their results in quantitative terms (replication of effects beyond significance, Open Science Collaboration, 2015 ), (ii) to compute power to future studies ( Lakens & Evers, 2014 ), and (iii) to aggregate results for meta-analyses whilst minimizing publication bias ( van Assen et al. , 2014 ).

[version 3; referees: 1 approved

Funding Statement

The author(s) declared that no grants were involved in supporting this work.

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Referee response for version 3

Dorothy vera margaret bishop.

1 Department of Experimental Psychology, University of Oxford, Oxford, UK

I can see from the history of this paper that the author has already been very responsive to reviewer comments, and that the process of revising has now been quite protracted.

That makes me reluctant to suggest much more, but I do see potential here for making the paper more impactful. So my overall view is that, once a few typos are fixed (see below), this could be published as is, but I think there is an issue with the potential readership and that further revision could overcome this.

I suspect my take on this is rather different from other reviewers, as I do not regard myself as a statistics expert, though I am on the more quantitative end of the continuum of psychologists and I try to keep up to date. I think I am quite close to the target readership , insofar as I am someone who was taught about statistics ages ago and uses stats a lot, but never got adequate training in the kinds of topic covered by this paper. The fact that I am aware of controversies around the interpretation of confidence intervals etc is simply because I follow some discussions of this on social media. I am therefore very interested to have a clear account of these issues.

This paper contains helpful information for someone in this position, but it is not always clear, and I felt the relevance of some of the content was uncertain. So here are some recommendations:

As one previous reviewer noted, it’s questionable that there is a need for a tutorial introduction, and the limited length of this article does not lend itself to a full explanation. So it might be better to just focus on explaining as clearly as possible the problems people have had in interpreting key concepts. I think a title that made it clear this was the content would be more appealing than the current one.
P 3, col 1, para 3, last sentence. Although statisticians always emphasise the arbitrary nature of p < .05, we all know that in practice authors who use other values are likely to have their analyses queried. I wondered whether it would be useful here to note that in some disciplines different cutoffs are traditional, e.g. particle physics. Or you could cite David Colquhoun’s paper in which he recommends using p < .001 ( http://rsos.royalsocietypublishing.org/content/1/3/140216) - just to be clear that the traditional p < .05 has been challenged.

What I can’t work out is how you would explain the alpha from Neyman-Pearson in the same way (though I can see from Figure 1 that with N-P you could test an alternative hypothesis, such as the idea that the coin would be heads 75% of the time).

‘By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot….’ have ‘In failing to reject, we do not assume that H0 is true; one cannot argue against a theory from a non-significant result.’

I felt most readers would be interested to read about tests of equivalence and Bayesian approaches, but many would be unfamiliar with these and might like to see an example of how they work in practice – if space permitted.

Confidence intervals: I simply could not understand the first sentence – I wondered what was meant by ‘builds’ here. I understand about difficulties in comparing CI across studies when sample sizes differ, but I did not find the last sentence on p 4 easy to understand.
P 5: The sentence starting: ‘The alpha value has the same interpretation’ was also hard to understand, especially the term ‘1-alpha CI’. Here too I felt some concrete illustration might be helpful to the reader. And again, I also found the reference to Bayesian intervals tantalising – I think many readers won’t know how to compute these and something like a figure comparing a traditional CI with a Bayesian interval and giving a source for those who want to read on would be very helpful. The reference to ‘credible intervals’ in the penultimate paragraph is very unclear and needs a supporting reference – most readers will not be familiar with this concept.

P 3, col 1, para 2, line 2; “allows us to compute”

P 3, col 2, para 2, ‘probability of replicating’

P 3, col 2, para 2, line 4 ‘informative about’

P 3, col 2, para 4, line 2 delete ‘of’

P 3, col 2, para 5, line 9 – ‘conditioned’ is either wrong or too technical here: would ‘based’ be acceptable as alternative wording

P 3, col 2, para 5, line 13 ‘This dichotomisation allows one to distinguish’

P 3, col 2, para 5, last sentence, delete ‘Alternatively’.

P 3, col 2, last para line 2 ‘first’

P 4, col 2, para 2, last sentence is hard to understand; not sure if this is better: ‘If sample sizes differ between studies, the distribution of CIs cannot be specified a priori’

P 5, col 1, para 2, ‘a pattern of order’ – I did not understand what was meant by this

P 5, col 1, para 2, last sentence unclear: possible rewording: “If the goal is to test the size of an effect then NHST is not the method of choice, since testing can only reject the null hypothesis.’ (??)

P 5, col 1, para 3, line 1 delete ‘that’

P 5, col 1, para 3, line 3 ‘on’ -> ‘by’

P 5, col 2, para 1, line 4 , rather than ‘Here I propose to adopt’ I suggest ‘I recommend adopting’

P 5, col 2, para 1, line 13 ‘with’ -> ‘by’

P 5, col 2, para 1 – recommend deleting last sentence

P 5, col 2, para 2, line 2 ‘consider’ -> ‘anticipate’

P 5, col 2, para 2, delete ‘should always be included’

P 5, col 2, para 2, ‘type one’ -> ‘Type I’

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.

The University of Edinburgh, UK

I wondered about changing the focus slightly and modifying the title to reflect this to say something like: Null hypothesis significance testing: a guide to commonly misunderstood concepts and recommendations for good practice

Thank you for the suggestion – you indeed saw the intention behind the ‘tutorial’ style of the paper.

P 3, col 1, para 3, last sentence. Although statisticians always emphasise the arbitrary nature of p < .05, we all know that in practice authors who use other values are likely to have their analyses queried. I wondered whether it would be useful here to note that in some disciplines different cutoffs are traditional, e.g. particle physics. Or you could cite David Colquhoun’s paper in which he recommends using p < .001 ( http://rsos.royalsocietypublishing.org/content/1/3/140216) - just to be clear that the traditional p < .05 has been challenged.

I have added a sentence on this citing Colquhoun 2014 and the new Benjamin 2017 on using .005.

I agree that this point is always hard to appreciate, especially because it seems like in practice it makes little difference. I added a paragraph but using reaction times rather than a coin toss – thanks for the suggestion.

Added an example based on new table 1, following figure 1 – giving CI, equivalence tests and Bayes Factor (with refs to easy to use tools)

Changed builds to constructs (this simply means they are something we build) and added that the implication that probability coverage is not warranty when sample size change, is that we cannot compare CI.

I changed ‘ i.e. we accept that 1-alpha CI are wrong in alpha percent of the times in the long run’ to ‘, ‘e.g. a 95% CI is wrong in 5% of the times in the long run (i.e. if we repeat the experiment many times).’ – for Bayesian intervals I simply re-cited Morey & Rouder, 2011.

It is not the CI cannot be specified, it’s that the interval is not predictive of anything anymore! I changed it to ‘If sample sizes, however, differ between studies, there is no warranty that a CI from one study will be true at the rate alpha in a different study, which implies that CI cannot be compared across studies at this is rarely the same sample sizes’

I added (i.e. establish that A > B) – we test that conditions are ordered, but without further specification of the probability of that effect nor its size

Yes it works – thx

P 5, col 2, para 2, ‘type one’ -> ‘Type I’

Typos fixed, and suggestions accepted – thanks for that.

Stephen J. Senn

1 Luxembourg Institute of Health, Strassen, L-1445, Luxembourg

The revisions are OK for me, and I have changed my status to Approved.

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Referee response for version 2

On the whole I think that this article is reasonable, my main reservation being that I have my doubts on whether the literature needs yet another tutorial on this subject.

A further reservation I have is that the author, following others, stresses what in my mind is a relatively unimportant distinction between the Fisherian and Neyman-Pearson (NP) approaches. The distinction stressed by many is that the NP approach leads to a dichotomy accept/reject based on probabilities established in advance, whereas the Fisherian approach uses tail area probabilities calculated from the observed statistic. I see this as being unimportant and not even true. Unless one considers that the person carrying out a hypothesis test (original tester) is mandated to come to a conclusion on behalf of all scientific posterity, then one must accept that any remote scientist can come to his or her conclusion depending on the personal type I error favoured. To operate the results of an NP test carried out by the original tester, the remote scientist then needs to know the p-value. The type I error rate is then compared to this to come to a personal accept or reject decision (1). In fact Lehmann (2), who was an important developer of and proponent of the NP system, describes exactly this approach as being good practice. (See Testing Statistical Hypotheses, 2nd edition P70). Thus using tail-area probabilities calculated from the observed statistics does not constitute an operational difference between the two systems.

A more important distinction between the Fisherian and NP systems is that the former does not use alternative hypotheses(3). Fisher's opinion was that the null hypothesis was more primitive than the test statistic but that the test statistic was more primitive than the alternative hypothesis. Thus, alternative hypotheses could not be used to justify choice of test statistic. Only experience could do that.

Further distinctions between the NP and Fisherian approach are to do with conditioning and whether a null hypothesis can ever be accepted.

I have one minor quibble about terminology. As far as I can see, the author uses the usual term 'null hypothesis' and the eccentric term 'nil hypothesis' interchangeably. It would be simpler if the latter were abandoned.

Referee response for version 1

Marcel alm van assen.

1 Department of Methodology and Statistics, Tilburgh University, Tilburg, Netherlands

Null hypothesis significance testing (NHST) is a difficult topic, with misunderstandings arising easily. Many texts, including basic statistics books, deal with the topic, and attempt to explain it to students and anyone else interested. I would refer to a good basic text book, for a detailed explanation of NHST, or to a specialized article when wishing an explaining the background of NHST. So, what is the added value of a new text on NHST? In any case, the added value should be described at the start of this text. Moreover, the topic is so delicate and difficult that errors, misinterpretations, and disagreements are easy. I attempted to show this by giving comments to many sentences in the text.

Abstract: “null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences to investigate if an effect is likely”. No, NHST is the method to test the hypothesis of no effect.

Intro: “Null hypothesis significance testing (NHST) is a method of statistical inference by which an observation is tested against a hypothesis of no effect or no relationship.” What is an ‘observation’? NHST is difficult to describe in one sentence, particularly here. I would skip this sentence entirely, here.

Section on Fisher; also explain the one-tailed test.

Section on Fisher; p(Obs|H0) does not reflect the verbal definition (the ‘or more extreme’ part).

Section on Fisher; use a reference and citation to Fisher’s interpretation of the p-value

Section on Fisher; “This was however only intended to be used as an indication that there is something in the data that deserves further investigation. The reason for this is that only H0 is tested whilst the effect under study is not itself being investigated.” First sentence, can you give a reference? Many people say a lot about Fisher’s intentions, but the good man is dead and cannot reply… Second sentence is a bit awkward, because the effect is investigated in a way, by testing the H0.

Section on p-value; Layout and structure can be improved greatly, by first again stating what the p-value is, and then statement by statement, what it is not, using separate lines for each statement. Consider adding that the p-value is randomly distributed under H0 (if all the assumptions of the test are met), and that under H1 the p-value is a function of population effect size and N; the larger each is, the smaller the p-value generally is.

Skip the sentence “If there is no effect, we should replicate the absence of effect with a probability equal to 1-p”. Not insightful, and you did not discuss the concept ‘replicate’ (and do not need to).

Skip the sentence “The total probability of false positives can also be obtained by aggregating results ( Ioannidis, 2005 ).” Not strongly related to p-values, and introduces unnecessary concepts ‘false positives’ (perhaps later useful) and ‘aggregation’.

Consider deleting; “If there is an effect however, the probability to replicate is a function of the (unknown) population effect size with no good way to know this from a single experiment ( Killeen, 2005 ).”

The following sentence; “ Finally, a (small) p-value is not an indication favouring a hypothesis . A low p-value indicates a misfit of the null hypothesis to the data and cannot be taken as evidence in favour of a specific alternative hypothesis more than any other possible alternatives such as measurement error and selection bias ( Gelman, 2013 ).” is surely not mainstream thinking about NHST; I would surely delete that sentence. In NHST, a p-value is used for testing the H0. Why did you not yet discuss significance level? Yes, before discussing what is not a p-value, I would explain NHST (i.e., what it is and how it is used).

Also the next sentence “The more (a priori) implausible the alternative hypothesis, the greater the chance that a finding is a false alarm ( Krzywinski & Altman, 2013 ; Nuzzo, 2014 ).“ is not fully clear to me. This is a Bayesian statement. In NHST, no likelihoods are attributed to hypotheses; the reasoning is “IF H0 is true, then…”.

Last sentence: “As Nickerson (2000) puts it ‘theory corroboration requires the testing of multiple predictions because the chance of getting statistically significant results for the wrong reasons in any given case is high’.” What is relation of this sentence to the contents of this section, precisely?

Next section: “For instance, we can estimate that the probability of a given F value to be in the critical interval [+2 +∞] is less than 5%” This depends on the degrees of freedom.

“When there is no effect (H0 is true), the erroneous rejection of H0 is known as type I error and is equal to the p-value.” Strange sentence. The Type I error is the probability of erroneously rejecting the H0 (so, when it is true). The p-value is … well, you explained it before; it surely does not equal the Type I error.

Consider adding a figure explaining the distinction between Fisher’s logic and that of Neyman and Pearson.

“When the test statistics falls outside the critical region(s)” What is outside?

“There is a profound difference between accepting the null hypothesis and simply failing to reject it ( Killeen, 2005 )” I agree with you, but perhaps you may add that some statisticians simply define “accept H0’” as obtaining a p-value larger than the significance level. Did you already discuss the significance level, and it’s mostly used values?

“To accept or reject equally the null hypothesis, Bayesian approaches ( Dienes, 2014 ; Kruschke, 2011 ) or confidence intervals must be used.” Is ‘reject equally’ appropriate English? Also using Cis, one cannot accept the H0.

Do you start discussing alpha only in the context of Cis?

“CI also indicates the precision of the estimate of effect size, but unless using a percentile bootstrap approach, they require assumptions about distributions which can lead to serious biases in particular regarding the symmetry and width of the intervals ( Wilcox, 2012 ).” Too difficult, using new concepts. Consider deleting.

“Assuming the CI (a)symmetry and width are correct, this gives some indication about the likelihood that a similar value can be observed in future studies, with 95% CI giving about 83% chance of replication success ( Lakens & Evers, 2014 ).” This statement is, in general, completely false. It very much depends on the sample sizes of both studies. If the replication study has a much, much, much larger N, then the probability that the original CI will contain the effect size of the replication approaches (1-alpha)*100%. If the original study has a much, much, much larger N, then the probability that the original Ci will contain the effect size of the replication study approaches 0%.

“Finally, contrary to p-values, CI can be used to accept H0. Typically, if a CI includes 0, we cannot reject H0. If a critical null region is specified rather than a single point estimate, for instance [-2 +2] and the CI is included within the critical null region, then H0 can be accepted. Importantly, the critical region must be specified a priori and cannot be determined from the data themselves.” No. H0 cannot be accepted with Cis.

“The (posterior) probability of an effect can however not be obtained using a frequentist framework.” Frequentist framework? You did not discuss that, yet.

“X% of times the CI obtained will contain the same parameter value”. The same? True, you mean?

“e.g. X% of the times the CI contains the same mean” I do not understand; which mean?

“The alpha value has the same interpretation as when using H0, i.e. we accept that 1-alpha CI are wrong in alpha percent of the times. “ What do you mean, CI are wrong? Consider rephrasing.

“To make a statement about the probability of a parameter of interest, likelihood intervals (maximum likelihood) and credibility intervals (Bayes) are better suited.” ML gives the likelihood of the data given the parameter, not the other way around.

“Many of the disagreements are not on the method itself but on its use.” Bayesians may disagree.

“If the goal is to establish the likelihood of an effect and/or establish a pattern of order, because both requires ruling out equivalence, then NHST is a good tool ( Frick, 1996 )” NHST does not provide evidence on the likelihood of an effect.

“If the goal is to establish some quantitative values, then NHST is not the method of choice.” P-values are also quantitative… this is not a precise sentence. And NHST may be used in combination with effect size estimation (this is even recommended by, e.g., the American Psychological Association (APA)).

“Because results are conditioned on H0, NHST cannot be used to establish beliefs.” It can reinforce some beliefs, e.g., if H0 or any other hypothesis, is true.

“To estimate the probability of a hypothesis, a Bayesian analysis is a better alternative.” It is the only alternative?

“Note however that even when a specific quantitative prediction from a hypothesis is shown to be true (typically testing H1 using Bayes), it does not prove the hypothesis itself, it only adds to its plausibility.” How can we show something is true?

I do not agree on the contents of the last section on ‘minimal reporting’. I prefer ‘optimal reporting’ instead, i.e., the reporting the information that is essential to the interpretation of the result, to any ready, which may have other goals than the writer of the article. This reporting includes, for sure, an estimate of effect size, and preferably a confidence interval, which is in line with recommendations of the APA.

I have read this submission. I believe that I have an appropriate level of expertise to state that I do not consider it to be of an acceptable scientific standard, for reasons outlined above.

The idea of this short review was to point to common interpretation errors (stressing again and again that we are under H0) being in using p-values or CI, and also proposing reporting practices to avoid bias. This is now stated at the end of abstract.

Regarding text books, it is clear that many fail to clearly distinguish Fisher/Pearson/NHST, see Glinet et al (2012) J. Exp Education 71, 83-92. If you have 1 or 2 in mind that you know to be good, I’m happy to include them.

I agree – yet people use it to investigate (not test) if an effect is likely. The issue here is wording. What about adding this distinction at the end of the sentence?: ‘null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences used to investigate if an effect is likely, even though it actually tests for the hypothesis of no effect’.

I think a definition is needed, as it offers a starting point. What about the following: ‘NHST is a method of statistical inference by which an experimental factor is tested against a hypothesis of no effect or no relationship based on a given observation’

The section on Fisher has been modified (more or less) as suggested: (1) avoiding talking about one or two tailed tests (2) updating for p(Obs≥t|H0) and (3) referring to Fisher more explicitly (ie pages from articles and book) ; I cannot tell his intentions but these quotes leave little space to alternative interpretations.

The reasoning here is as you state yourself, part 1: ‘a p-value is used for testing the H0; and part 2: ‘no likelihoods are attributed to hypotheses’ it follows we cannot favour a hypothesis. It might seems contentious but this is the case that all we can is to reject the null – how could we favour a specific alternative hypothesis from there? This is explored further down the manuscript (and I now point to that) – note that we do not need to be Bayesian to favour a specific H1, all I’m saying is this cannot be attained with a p-value.

The point was to emphasise that a p value is not there to tell us a given H1 is true and can only be achieved through multiple predictions and experiments. I deleted it for clarity.

This sentence has been removed

Indeed, you are right and I have modified the text accordingly. When there is no effect (H0 is true), the erroneous rejection of H0 is known as type 1 error. Importantly, the type 1 error rate, or alpha value is determined a priori. It is a common mistake but the level of significance (for a given sample) is not the same as the frequency of acceptance alpha found on repeated sampling (Fisher, 1955).

A figure is now presented – with levels of acceptance, critical region, level of significance and p-value.

I should have clarified further here – as I was having in mind tests of equivalence. To clarify, I simply states now: ‘To accept the null hypothesis, tests of equivalence or Bayesian approaches must be used.’

It is now presented in the paragraph before.

Yes, you are right, I completely overlooked this problem. The corrected sentence (with more accurate ref) is now “Assuming the CI (a)symmetry and width are correct, this gives some indication about the likelihood that a similar value can be observed in future studies. For future studies of the same sample size, 95% CI giving about 83% chance of replication success (Cumming and Mallardet, 2006). If sample sizes differ between studies, CI do not however warranty any a priori coverage”.

Again, I had in mind equivalence testing, but in both cases you are right we can only reject and I therefore removed that sentence.

Yes, p-values must be interpreted in context with effect size, but this is not what people do. The point here is to be pragmatic, does and don’t. The sentence was changed.

Not for testing, but for probability, I am not aware of anything else.

Cumulative evidence is, in my opinion, the only way to show it. Even in hard science like physics multiple experiments. In the recent CERN study on finding Higgs bosons, 2 different and complementary experiments ran in parallel – and the cumulative evidence was taken as a proof of the true existence of Higgs bosons.

Daniel Lakens

1 School of Innovation Sciences, Eindhoven University of Technology, Eindhoven, Netherlands

I appreciate the author's attempt to write a short tutorial on NHST. Many people don't know how to use it, so attempts to educate people are always worthwhile. However, I don't think the current article reaches it's aim. For one, I think it might be practically impossible to explain a lot in such an ultra short paper - every section would require more than 2 pages to explain, and there are many sections. Furthermore, there are some excellent overviews, which, although more extensive, are also much clearer (e.g., Nickerson, 2000 ). Finally, I found many statements to be unclear, and perhaps even incorrect (noted below). Because there is nothing worse than creating more confusion on such a topic, I have extremely high standards before I think such a short primer should be indexed. I note some examples of unclear or incorrect statements below. I'm sorry I can't make a more positive recommendation.

“investigate if an effect is likely” – ambiguous statement. I think you mean, whether the observed DATA is probable, assuming there is no effect?

The Fisher (1959) reference is not correct – Fischer developed his method much earlier.

“This p-value thus reflects the conditional probability of achieving the observed outcome or larger, p(Obs|H0)” – please add 'assuming the null-hypothesis is true'.

“p(Obs|H0)” – explain this notation for novices.

“Following Fisher, the smaller the p-value, the greater the likelihood that the null hypothesis is false.” This is wrong, and any statement about this needs to be much more precise. I would suggest direct quotes.

“there is something in the data that deserves further investigation” –unclear sentence.

“The reason for this” – unclear what ‘this’ refers to.

“ not the probability of the null hypothesis of being true, p(H0)” – second of can be removed?

“Any interpretation of the p-value in relation to the effect under study (strength, reliability, probability) is indeed

wrong, since the p-value is conditioned on H0” - incorrect. A big problem is that it depends on the sample size, and that the probability of a theory depends on the prior.

“If there is no effect, we should replicate the absence of effect with a probability equal to 1-p.” I don’t understand this, but I think it is incorrect.

“The total probability of false positives can also be obtained by aggregating results (Ioannidis, 2005).” Unclear, and probably incorrect.

“By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot, from a nonsignificant result, argue against a theory” – according to which theory? From a NP perspective, you can ACT as if the theory is false.

“(Lakens & Evers, 2014”) – we are not the original source, which should be cited instead.

“ Typically, if a CI includes 0, we cannot reject H0.” - when would this not be the case? This assumes a CI of 1-alpha.

“If a critical null region is specified rather than a single point estimate, for instance [-2 +2] and the CI is included within the critical null region, then H0 can be accepted.” – you mean practically, or formally? I’m pretty sure only the former.

The section on ‘The (correct) use of NHST’ seems to conclude only Bayesian statistics should be used. I don’t really agree.

“ we can always argue that effect size, power, etc. must be reported.” – which power? Post-hoc power? Surely not? Other types are unknown. So what do you mean?

The recommendation on what to report remains vague, and it is unclear why what should be reported.

This sentence was changed, following as well the other reviewer, to ‘null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences to investigate if an effect is likely, even though it actually tests whether the observed data are probable, assuming there is no effect’

Changed, refers to Fisher 1925

I changed a little the sentence structure, which should make explicit that this is the condition probability.

This has been changed to ‘[…] to decide whether the evidence is worth additional investigation and/or replication (Fisher, 1971 p13)’

my mistake – the sentence structure is now ‘ not the probability of the null hypothesis p(H0), of being true,’ ; hope this makes more sense (and this way refers back to p(Obs>t|H0)

Fair enough – my point was to stress the fact that p value and effect size or H1 have very little in common, but yes that the part in common has to do with sample size. I left the conditioning on H0 but also point out the dependency on sample size.

The whole paragraph was changed to reflect a more philosophical take on scientific induction/reasoning. I hope this is clearer.

Changed to refer to equivalence testing

I rewrote this, as to show frequentist analysis can be used - I’m trying to sell Bayes more than any other approach.

I’m arguing we should report it all, that’s why there is no exhausting list – I can if needed.

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Null Hypothesis

Null Hypothesis , often denoted as H 0, is a foundational concept in statistical hypothesis testing. It represents an assumption that no significant difference, effect, or relationship exists between variables within a population. It serves as a baseline assumption, positing no observed change or effect occurring. The null is t he truth or falsity of an idea in analysis.

In this article, we will discuss the null hypothesis in detail, along with some solved examples and questions on the null hypothesis.

Table of Content

What is Null Hypothesis?

Null hypothesis symbol, formula of null hypothesis, types of null hypothesis, null hypothesis examples, principle of null hypothesis, how do you find null hypothesis, null hypothesis in statistics, null hypothesis and alternative hypothesis, null hypothesis and alternative hypothesis examples, null hypothesis – practice problems.

Null Hypothesis in statistical analysis suggests the absence of statistical significance within a specific set of observed data. Hypothesis testing, using sample data, evaluates the validity of this hypothesis. Commonly denoted as H 0 or simply “null,” it plays an important role in quantitative analysis, examining theories related to markets, investment strategies, or economies to determine their validity.

Null Hypothesis Meaning

Null Hypothesis represents a default position, often suggesting no effect or difference, against which researchers compare their experimental results. The Null Hypothesis, often denoted as H 0 asserts a default assumption in statistical analysis. It posits no significant difference or effect, serving as a baseline for comparison in hypothesis testing.

The null Hypothesis is represented as H 0 , the Null Hypothesis symbolizes the absence of a measurable effect or difference in the variables under examination.

Certainly, a simple example would be asserting that the mean score of a group is equal to a specified value like stating that the average IQ of a population is 100.

The Null Hypothesis is typically formulated as a statement of equality or absence of a specific parameter in the population being studied. It provides a clear and testable prediction for comparison with the alternative hypothesis. The formulation of the Null Hypothesis typically follows a concise structure, stating the equality or absence of a specific parameter in the population.

Mean Comparison (Two-sample t-test)

H 0 : μ 1 = μ 2

This asserts that there is no significant difference between the means of two populations or groups.

Proportion Comparison

H 0 : p 1 − p 2 = 0

This suggests no significant difference in proportions between two populations or conditions.

Equality in Variance (F-test in ANOVA)

H 0 : σ 1 = σ 2

This states that there’s no significant difference in variances between groups or populations.

Independence (Chi-square Test of Independence):

H 0 : Variables are independent

This asserts that there’s no association or relationship between categorical variables.

Null Hypotheses vary including simple and composite forms, each tailored to the complexity of the research question. Understanding these types is pivotal for effective hypothesis testing.

Equality Null Hypothesis (Simple Null Hypothesis)

The Equality Null Hypothesis, also known as the Simple Null Hypothesis, is a fundamental concept in statistical hypothesis testing that assumes no difference, effect or relationship between groups, conditions or populations being compared.

Non-Inferiority Null Hypothesis

In some studies, the focus might be on demonstrating that a new treatment or method is not significantly worse than the standard or existing one.

Superiority Null Hypothesis

The concept of a superiority null hypothesis comes into play when a study aims to demonstrate that a new treatment, method, or intervention is significantly better than an existing or standard one.

Independence Null Hypothesis

In certain statistical tests, such as chi-square tests for independence, the null hypothesis assumes no association or independence between categorical variables.

Homogeneity Null Hypothesis

In tests like ANOVA (Analysis of Variance), the null hypothesis suggests that there’s no difference in population means across different groups.

Medicine: Null Hypothesis: “No significant difference exists in blood pressure levels between patients given the experimental drug versus those given a placebo.”
Education: Null Hypothesis: “There’s no significant variation in test scores between students using a new teaching method and those using traditional teaching.”
Economics: Null Hypothesis: “There’s no significant change in consumer spending pre- and post-implementation of a new taxation policy.”
Environmental Science: Null Hypothesis: “There’s no substantial difference in pollution levels before and after a water treatment plant’s establishment.”

The principle of the null hypothesis is a fundamental concept in statistical hypothesis testing. It involves making an assumption about the population parameter or the absence of an effect or relationship between variables.

In essence, the null hypothesis (H 0 ) proposes that there is no significant difference, effect, or relationship between variables. It serves as a starting point or a default assumption that there is no real change, no effect or no difference between groups or conditions.

The null hypothesis is usually formulated to be tested against an alternative hypothesis (H 1 or H [Tex]\alpha [/Tex] ) which suggests that there is an effect, difference or relationship present in the population.

Null Hypothesis Rejection

Rejecting the Null Hypothesis occurs when statistical evidence suggests a significant departure from the assumed baseline. It implies that there is enough evidence to support the alternative hypothesis, indicating a meaningful effect or difference. Null Hypothesis rejection occurs when statistical evidence suggests a deviation from the assumed baseline, prompting a reconsideration of the initial hypothesis.

Identifying the Null Hypothesis involves defining the status quotient, asserting no effect and formulating a statement suitable for statistical analysis.

When is Null Hypothesis Rejected?

The Null Hypothesis is rejected when statistical tests indicate a significant departure from the expected outcome, leading to the consideration of alternative hypotheses. It occurs when statistical evidence suggests a deviation from the assumed baseline, prompting a reconsideration of the initial hypothesis.

In statistical hypothesis testing, researchers begin by stating the null hypothesis, often based on theoretical considerations or previous research. The null hypothesis is then tested against an alternative hypothesis (Ha), which represents the researcher’s claim or the hypothesis they seek to support.

The process of hypothesis testing involves collecting sample data and using statistical methods to assess the likelihood of observing the data if the null hypothesis were true. This assessment is typically done by calculating a test statistic, which measures the difference between the observed data and what would be expected under the null hypothesis.

In the realm of hypothesis testing, the null hypothesis (H 0 ) and alternative hypothesis (H₁ or Ha) play critical roles. The null hypothesis generally assumes no difference, effect, or relationship between variables, suggesting that any observed change or effect is due to random chance. Its counterpart, the alternative hypothesis, asserts the presence of a significant difference, effect, or relationship between variables, challenging the null hypothesis. These hypotheses are formulated based on the research question and guide statistical analyses.

Difference Between Null Hypothesis and Alternative Hypothesis

The null hypothesis (H 0 ) serves as the baseline assumption in statistical testing, suggesting no significant effect, relationship, or difference within the data. It often proposes that any observed change or correlation is merely due to chance or random variation. Conversely, the alternative hypothesis (H 1 or Ha) contradicts the null hypothesis, positing the existence of a genuine effect, relationship or difference in the data. It represents the researcher’s intended focus, seeking to provide evidence against the null hypothesis and support for a specific outcome or theory. These hypotheses form the crux of hypothesis testing, guiding the assessment of data to draw conclusions about the population being studied.


Criteria	Null Hypothesis	Alternative Hypothesis
Definition	Assumes no effect or difference	Asserts a specific effect or difference
Symbol	H	H (or Ha)
Formulation	States equality or absence of parameter	States a specific value or relationship
Testing Outcome	Rejected if evidence of a significant effect	Accepted if evidence supports the hypothesis

Let’s envision a scenario where a researcher aims to examine the impact of a new medication on reducing blood pressure among patients. In this context:

Null Hypothesis (H 0 ): “The new medication does not produce a significant effect in reducing blood pressure levels among patients.”

Alternative Hypothesis (H 1 or Ha): “The new medication yields a significant effect in reducing blood pressure levels among patients.”

The null hypothesis implies that any observed alterations in blood pressure subsequent to the medication’s administration are a result of random fluctuations rather than a consequence of the medication itself. Conversely, the alternative hypothesis contends that the medication does indeed generate a meaningful alteration in blood pressure levels, distinct from what might naturally occur or by random chance.

Summary – Null Hypothesis and Alternative Hypothesis

The null hypothesis (H 0 ) and alternative hypothesis (H a ) are fundamental concepts in statistical hypothesis testing. The null hypothesis represents the default assumption, stating that there is no significant effect, difference, or relationship between variables. It serves as the baseline against which the alternative hypothesis is tested. In contrast, the alternative hypothesis represents the researcher’s hypothesis or the claim to be tested, suggesting that there is a significant effect, difference, or relationship between variables. The relationship between the null and alternative hypotheses is such that they are complementary, and statistical tests are conducted to determine whether the evidence from the data is strong enough to reject the null hypothesis in favor of the alternative hypothesis. This decision is based on the strength of the evidence and the chosen level of significance. Ultimately, the choice between the null and alternative hypotheses depends on the specific research question and the direction of the effect being investigated.

FAQs on Null Hypothesis

What does null hypothesis stands for.

The null hypothesis, denoted as H 0 , is a fundamental concept in statistics used for hypothesis testing. It represents the statement that there is no effect or no difference, and it is the hypothesis that the researcher typically aims to provide evidence against.

How to Form a Null Hypothesis?

A null hypothesis is formed based on the assumption that there is no significant difference or effect between the groups being compared or no association between variables being tested. It often involves stating that there is no relationship, no change, or no effect in the population being studied.

When Do we reject the Null Hypothesis?

In statistical hypothesis testing, if the p-value (the probability of obtaining the observed results) is lower than the chosen significance level (commonly 0.05), we reject the null hypothesis. This suggests that the data provides enough evidence to refute the assumption made in the null hypothesis.

What is a Null Hypothesis in Research?

In research, the null hypothesis represents the default assumption or position that there is no significant difference or effect. Researchers often try to test this hypothesis by collecting data and performing statistical analyses to see if the observed results contradict the assumption.

What Are Alternative and Null Hypotheses?

The null hypothesis (H0) is the default assumption that there is no significant difference or effect. The alternative hypothesis (H1 or Ha) is the opposite, suggesting there is a significant difference, effect or relationship.

What Does it Mean to Reject the Null Hypothesis?

Rejecting the null hypothesis implies that there is enough evidence in the data to support the alternative hypothesis. In simpler terms, it suggests that there might be a significant difference, effect or relationship between the groups or variables being studied.

How to Find Null Hypothesis?

Formulating a null hypothesis often involves considering the research question and assuming that no difference or effect exists. It should be a statement that can be tested through data collection and statistical analysis, typically stating no relationship or no change between variables or groups.

How is Null Hypothesis denoted?

The null hypothesis is commonly symbolized as H 0 in statistical notation.

What is the Purpose of the Null hypothesis in Statistical Analysis?

The null hypothesis serves as a starting point for hypothesis testing, enabling researchers to assess if there’s enough evidence to reject it in favor of an alternative hypothesis.

What happens if we Reject the Null hypothesis?

Rejecting the null hypothesis implies that there is sufficient evidence to support an alternative hypothesis, suggesting a significant effect or relationship between variables.

What are Test for Null Hypothesis?

Various statistical tests, such as t-tests or chi-square tests, are employed to evaluate the validity of the Null Hypothesis in different scenarios.

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Choosing the Right Statistical Test | Types & Examples

Choosing the Right Statistical Test | Types & Examples

Published on January 28, 2020 by Rebecca Bevans . Revised on June 22, 2023.

Statistical tests are used in hypothesis testing . They can be used to:

determine whether a predictor variable has a statistically significant relationship with an outcome variable.
estimate the difference between two or more groups.

Statistical tests assume a null hypothesis of no relationship or no difference between groups. Then they determine whether the observed data fall outside of the range of values predicted by the null hypothesis.

If you already know what types of variables you’re dealing with, you can use the flowchart to choose the right statistical test for your data.

Statistical tests flowchart

What does a statistical test do, when to perform a statistical test, choosing a parametric test: regression, comparison, or correlation, choosing a nonparametric test, flowchart: choosing a statistical test, other interesting articles, frequently asked questions about statistical tests.

Statistical tests work by calculating a test statistic – a number that describes how much the relationship between variables in your test differs from the null hypothesis of no relationship.

It then calculates a p value (probability value). The p -value estimates how likely it is that you would see the difference described by the test statistic if the null hypothesis of no relationship were true.

If the value of the test statistic is more extreme than the statistic calculated from the null hypothesis, then you can infer a statistically significant relationship between the predictor and outcome variables.

If the value of the test statistic is less extreme than the one calculated from the null hypothesis, then you can infer no statistically significant relationship between the predictor and outcome variables.

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You can perform statistical tests on data that have been collected in a statistically valid manner – either through an experiment , or through observations made using probability sampling methods .

For a statistical test to be valid , your sample size needs to be large enough to approximate the true distribution of the population being studied.

To determine which statistical test to use, you need to know:

whether your data meets certain assumptions.
the types of variables that you’re dealing with.

Statistical assumptions

Statistical tests make some common assumptions about the data they are testing:

Independence of observations (a.k.a. no autocorrelation): The observations/variables you include in your test are not related (for example, multiple measurements of a single test subject are not independent, while measurements of multiple different test subjects are independent).
Homogeneity of variance : the variance within each group being compared is similar among all groups. If one group has much more variation than others, it will limit the test’s effectiveness.
Normality of data : the data follows a normal distribution (a.k.a. a bell curve). This assumption applies only to quantitative data .

If your data do not meet the assumptions of normality or homogeneity of variance, you may be able to perform a nonparametric statistical test , which allows you to make comparisons without any assumptions about the data distribution.

If your data do not meet the assumption of independence of observations, you may be able to use a test that accounts for structure in your data (repeated-measures tests or tests that include blocking variables).

Types of variables

The types of variables you have usually determine what type of statistical test you can use.

Quantitative variables represent amounts of things (e.g. the number of trees in a forest). Types of quantitative variables include:

Continuous (aka ratio variables): represent measures and can usually be divided into units smaller than one (e.g. 0.75 grams).
Discrete (aka integer variables): represent counts and usually can’t be divided into units smaller than one (e.g. 1 tree).

Categorical variables represent groupings of things (e.g. the different tree species in a forest). Types of categorical variables include:

Ordinal : represent data with an order (e.g. rankings).
Nominal : represent group names (e.g. brands or species names).
Binary : represent data with a yes/no or 1/0 outcome (e.g. win or lose).

Choose the test that fits the types of predictor and outcome variables you have collected (if you are doing an experiment , these are the independent and dependent variables ). Consult the tables below to see which test best matches your variables.

Parametric tests usually have stricter requirements than nonparametric tests, and are able to make stronger inferences from the data. They can only be conducted with data that adheres to the common assumptions of statistical tests.

The most common types of parametric test include regression tests, comparison tests, and correlation tests.

Regression tests

Regression tests look for cause-and-effect relationships . They can be used to estimate the effect of one or more continuous variables on another variable.

	Predictor variable	Outcome variable	Research question example
			What is the effect of income on longevity?
			What is the effect of income and minutes of exercise per day on longevity?
Logistic regression			What is the effect of drug dosage on the survival of a test subject?

Comparison tests

Comparison tests look for differences among group means . They can be used to test the effect of a categorical variable on the mean value of some other characteristic.

T-tests are used when comparing the means of precisely two groups (e.g., the average heights of men and women). ANOVA and MANOVA tests are used when comparing the means of more than two groups (e.g., the average heights of children, teenagers, and adults).

	Predictor variable	Outcome variable	Research question example
Paired t-test			What is the effect of two different test prep programs on the average exam scores for students from the same class?
Independent t-test			What is the difference in average exam scores for students from two different schools?
ANOVA			What is the difference in average pain levels among post-surgical patients given three different painkillers?
MANOVA			What is the effect of flower species on petal length, petal width, and stem length?

Correlation tests

Correlation tests check whether variables are related without hypothesizing a cause-and-effect relationship.

These can be used to test whether two variables you want to use in (for example) a multiple regression test are autocorrelated.

	Variables	Research question example
Pearson’s		How are latitude and temperature related?

Non-parametric tests don’t make as many assumptions about the data, and are useful when one or more of the common statistical assumptions are violated. However, the inferences they make aren’t as strong as with parametric tests.

	Predictor variable	Outcome variable	Use in place of…
Spearman’s
			Pearson’s
Sign test			One-sample -test
Kruskal–Wallis			ANOVA
ANOSIM			MANOVA
Wilcoxon Rank-Sum test			Independent t-test
Wilcoxon Signed-rank test			Paired t-test

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This flowchart helps you choose among parametric tests. For nonparametric alternatives, check the table above.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

Normal distribution
Descriptive statistics
Measures of central tendency
Correlation coefficient
Null hypothesis

Methodology

Cluster sampling
Stratified sampling
Types of interviews
Cohort study
Thematic analysis

Research bias

Implicit bias
Cognitive bias
Survivorship bias
Availability heuristic
Nonresponse bias
Regression to the mean

Statistical tests commonly assume that:

the data are normally distributed
the groups that are being compared have similar variance
the data are independent

If your data does not meet these assumptions you might still be able to use a nonparametric statistical test , which have fewer requirements but also make weaker inferences.

A test statistic is a number calculated by a statistical test . It describes how far your observed data is from the null hypothesis of no relationship between variables or no difference among sample groups.

The test statistic tells you how different two or more groups are from the overall population mean , or how different a linear slope is from the slope predicted by a null hypothesis . Different test statistics are used in different statistical tests.

Statistical significance is a term used by researchers to state that it is unlikely their observations could have occurred under the null hypothesis of a statistical test . Significance is usually denoted by a p -value , or probability value.

Statistical significance is arbitrary – it depends on the threshold, or alpha value, chosen by the researcher. The most common threshold is p < 0.05, which means that the data is likely to occur less than 5% of the time under the null hypothesis .

When the p -value falls below the chosen alpha value, then we say the result of the test is statistically significant.

Quantitative variables are any variables where the data represent amounts (e.g. height, weight, or age).

Categorical variables are any variables where the data represent groups. This includes rankings (e.g. finishing places in a race), classifications (e.g. brands of cereal), and binary outcomes (e.g. coin flips).

You need to know what type of variables you are working with to choose the right statistical test for your data and interpret your results .

Discrete and continuous variables are two types of quantitative variables :

Discrete variables represent counts (e.g. the number of objects in a collection).
Continuous variables represent measurable amounts (e.g. water volume or weight).

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What is: Null Distribution

What is null distribution.

Null distribution refers to the probability distribution of a statistic under the null hypothesis. In hypothesis testing, the null hypothesis typically posits that there is no effect or no difference between groups. The null distribution serves as a benchmark against which the observed data can be compared to determine the likelihood of obtaining the observed results if the null hypothesis were true. Understanding null distribution is crucial for interpreting p-values and making informed decisions based on statistical tests.

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Importance of Null Distribution in Hypothesis Testing

The significance of null distribution lies in its role in hypothesis testing. It provides a framework for determining whether the observed data deviates significantly from what would be expected under the null hypothesis. By comparing the test statistic derived from the sample data to the null distribution, researchers can assess the probability of observing such extreme results purely by chance. This comparison is fundamental in deciding whether to reject or fail to reject the null hypothesis.

Types of Null Distributions

There are various types of null distributions, each corresponding to different statistical tests. For example, the normal distribution is often used in tests like the t-test when sample sizes are large enough. In contrast, the binomial distribution is applicable in tests involving proportions. Understanding the appropriate null distribution for a given statistical test is essential for accurate hypothesis testing and interpretation of results.

Constructing a Null Distribution

Constructing a null distribution typically involves simulating data under the null hypothesis. This can be achieved through resampling techniques such as bootstrapping or permutation tests. By generating a large number of samples from the null hypothesis, researchers can create an empirical null distribution that reflects the expected variability of the statistic. This empirical approach is particularly useful when the theoretical distribution is complex or unknown.

Null Distribution and P-Values

P-values are derived from the null distribution and represent the probability of observing a test statistic as extreme as, or more extreme than, the one calculated from the sample data, assuming the null hypothesis is true. A low p-value indicates that the observed data is unlikely under the null hypothesis, leading researchers to consider rejecting it. Thus, the relationship between null distribution and p-values is integral to the process of statistical inference.

Limitations of Null Distribution

While null distribution is a powerful concept in statistics, it has its limitations. One major limitation is the assumption that the null hypothesis is true, which may not always be the case. Additionally, the reliance on p-values for decision-making can lead to misinterpretation of results, especially in the context of multiple comparisons. Researchers must be cautious in their application of null distribution and consider alternative approaches to hypothesis testing.

Applications of Null Distribution

Null distribution is widely used across various fields, including psychology, medicine, and social sciences, to test hypotheses and draw conclusions from data. For instance, in clinical trials, researchers use null distribution to evaluate the efficacy of new treatments compared to standard care. In social sciences, null distribution helps assess the impact of interventions on behavioral outcomes. Its versatility makes it an essential tool in empirical research.

Visualizing Null Distribution

Visualizing null distribution can enhance understanding and interpretation of statistical results. Common methods include histograms, density plots, and Q-Q plots, which illustrate the shape and spread of the null distribution. By overlaying the observed test statistic on these visualizations, researchers can intuitively assess how extreme their results are in relation to the null distribution, aiding in the decision-making process.

Conclusion on Null Distribution

In summary, null distribution is a foundational concept in statistics that underpins hypothesis testing. Its role in determining the likelihood of observing data under the null hypothesis is critical for making informed decisions based on empirical evidence. By understanding the nuances of null distribution, researchers can enhance the rigor and validity of their statistical analyses.

COMMENTS

Null Hypothesis: Definition, Rejecting & Examples
The null hypothesis in statistics states that there is no difference between groups or no relationship between variables. It is one of two mutually exclusive hypotheses about a population in a hypothesis test. When your sample contains sufficient evidence, you can reject the null and conclude that the effect is statistically significant.
Null & Alternative Hypotheses
The null hypothesis (H0) answers "No, there's no effect in the population.". The alternative hypothesis (Ha) answers "Yes, there is an effect in the population.". The null and alternative are always claims about the population. That's because the goal of hypothesis testing is to make inferences about a population based on a sample.
Test Statistic: Definition, Types & Formulas
When your test statistic indicates a sufficiently large incompatibility with the null hypothesis, you can reject the null and state that your results are statistically significant—your data support the notion that the sample effect exists in the population. To use a test statistic to evaluate statistical significance, you either compare it to a critical value or use it to calculate the p-value.
Hypothesis Testing
Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.
Test statistics
The test statistic is a number calculated from a statistical test of a hypothesis. It shows how closely your observed data match the distribution expected under the null hypothesis of that statistical test. The test statistic is used to calculate the p value of your results, helping to decide whether to reject your null hypothesis.
What Is The Null Hypothesis & When To Reject It
We reject the null hypothesis when the data provide strong enough evidence to conclude that it is likely incorrect. This often occurs when the p-value (probability of observing the data given the null hypothesis is true) is below a predetermined significance level.
Null hypothesis
Basic definitions. The null hypothesis and the alternative hypothesis are types of conjectures used in statistical tests to make statistical inferences, which are formal methods of reaching conclusions and separating scientific claims from statistical noise. The statement being tested in a test of statistical significance is called the null ...
9.1: Null and Alternative Hypotheses
Learn how to formulate and test null and alternative hypotheses in statistics with examples and exercises from this LibreTexts course.
9.1 Null and Alternative Hypotheses
The actual test begins by considering two hypotheses. They are called the null hypothesis and the alternative hypothesis. These hypotheses contain oppos...
Null and Alternative Hypotheses
The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test: Null hypothesis (H0): There's no effect in the population. Alternative hypothesis (HA): There's an effect in the population. The effect is usually the effect of the independent variable on the dependent ...
Understanding Null Hypothesis Testing
A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the p value. A low p value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high p value means that the sample ...
9.1: Introduction to Hypothesis Testing
In hypothesis testing, the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis.
Null Hypothesis Definition and Examples, How to State
Null Hypothesis Overview The null hypothesis, H 0 is the commonly accepted fact; it is the opposite of the alternate hypothesis. Researchers work to reject, nullify or disprove the null hypothesis. Researchers come up with an alternate hypothesis, one that they think explains a phenomenon, and then work to reject the null hypothesis. Read on or watch the video for more information.
16.3: The Process of Null Hypothesis Testing
16.3.4 Step 4: Fit a model to the data and compute a test statistic For step 4, we want to use the data to compute a statistic that will ultimately let us decide whether the null hypothesis is rejected or not. To do this, the model needs to quantify the amount of evidence in favor of the alternative hypothesis, relative to the variability in the data. Thus we can think of the test statistic as ...
How to Write a Null Hypothesis (5 Examples)
How to Write a Null Hypothesis (5 Examples) A hypothesis test uses sample data to determine whether or not some claim about a population parameter is true. Whenever we perform a hypothesis test, we always write a null hypothesis and an alternative hypothesis, which take the following forms: H0 (Null Hypothesis): Population parameter =, ≤, ≥ ...
Null Hypothesis Definition and Examples
Null Hypothesis Examples. "Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a ...
13.1 Understanding Null Hypothesis Testing
The Logic of Null Hypothesis Testing Null hypothesis testing is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the null hypothesis (often symbolized H0 and read as "H-naught"). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling ...
How to Formulate a Null Hypothesis (With Examples)
The null hypothesis is among the easiest hypothesis to test using statistical analysis, making it perhaps the most valuable hypothesis for the scientific method. By evaluating a null hypothesis in addition to another hypothesis, researchers can support their conclusions with a higher level of confidence. Below are examples of how you might formulate a null hypothesis to fit certain questions.
1.2
Step 1: State the Null Hypothesis. The null hypothesis can be thought of as the opposite of the "guess" the researchers made. In the example presented in the previous section, the biologist "guesses" plant height will be different for the various fertilizers. So the null hypothesis would be that there will be no difference among the groups of ...
Null hypothesis significance testing: a short tutorial
Although thoroughly criticized, null hypothesis significance testing (NHST) remains the statistical method of choice used to provide evidence for an effect, in biological, biomedical and social sciences. In this short tutorial, I first summarize the concepts behind the method, distinguishing test of significance (Fisher) and test of acceptance ...
Null Hypothesis Statistical Testing (NHST)
Null Hypothesis Statistical Testing (NHST) Null Hypothesis Significance Testing (NHST) is a common statistical test to see if your research findings are statistically interesting. Its usefulness is sometimes challenged, particularly because NHST relies on p values, which are sporadically under fire from statisticians.
Null Hypothesis
Null hypothesis, often denoted as H0, is a foundational concept in statistical hypothesis testing. It represents an assumption that no significant difference, effect, or relationship exists between variables within a population. Learn more about Null Hypothesis, its formula, symbol and example in this article
Choosing the Right Statistical Test
What does a statistical test do? Statistical tests work by calculating a test statistic - a number that describes how much the relationship between variables in your test differs from the null hypothesis of no relationship. It then calculates a p value (probability value).
What is: Null Distribution
The significance of null distribution lies in its role in hypothesis testing. It provides a framework for determining whether the observed data deviates significantly from what would be expected under the null hypothesis. By comparing the test statistic derived from the sample data to the null distribution, researchers can assess the ...
8.2: The controversy over proper hypothesis testing
Confusingly, however, you cannot interpret the p-value as telling you the probability (how likely) that the null hypothesis is true. If, however, the test statistic is less than the critical value, then the conclusion is that the null hypothesis is to be provisionally accepted. The test statistic can be assigned a probability or p-value.
Kolmogorov-Smirnov test
Illustration of the Kolmogorov-Smirnov statistic. The red line is a model CDF, the blue line is an empirical CDF, and the black arrow is the KS statistic.. Kolmogorov-Smirnov test (K-S test or KS test) is a nonparametric test of the equality of continuous (or discontinuous, see Section 2.2), one-dimensional probability distributions that can be used to test whether a sample came from a ...

Test Statistic: Definition, Types & Formulas

What is a Test Statistic?

How to Find Test Statistics

Formulas for Test Statistics

Understanding the Null Values and the Test Statistic Formulas

T-Tests, Null = 0

F-tests including ANOVA, Null = 1

Chi-squared Tests, Null = 0

Interpreting Test Statistics

Sampling Distributions for Test Statistics

Example of a Test Statistic in a Sampling Distribution

Test Statistics and Critical Values

Using Test Statistics to Find P-values

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What is The Null Hypothesis & When Do You Reject The Null Hypothesis

How to Write a Null Hypothesis

For example, if studying the impact of exercise on weight loss, your null hypothesis might be:

Examples of Null Hypotheses

When Do We Reject The Null Hypothesis?

Why Do We Never Accept The Null Hypothesis?

Why Do We Use The Null Hypothesis?

Purpose of a Null Hypothesis

Do you always need both a Null Hypothesis and an Alternative Hypothesis?

What is the difference between a null hypothesis and an alternative hypothesis?

What are some problems with the null hypothesis?

Why can a null hypothesis not be accepted?

Is a null hypothesis directional or non-directional?

9.1 Null and Alternative Hypotheses

Example 9.1

Example 9.2

Example 9.3

Example 9.4

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Null and Alternative Hypotheses | Definitions & Examples

Table of contents

Examples of null hypotheses

Examples of alternative hypotheses

Test-specific

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Understanding Null Hypothesis Testing

The Purpose of Null Hypothesis Testing

The Logic of Null Hypothesis Testing

Role of Sample Size and Relationship Strength

Statistical Significance Versus Practical Significance

Long Descriptions

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Null Hypothesis Definition and Examples, How to State

Why is it Called the “Null”?

Why Do I need to Test it? Why not just prove an alternate one?

How to State the Null Hypothesis from a Word Problem

How to State the Null Hypothesis: Part Two

13.1 Understanding Null Hypothesis Testing

The Purpose of Null Hypothesis Testing

The Logic of Null Hypothesis Testing

The Misunderstood p Value

Role of Sample Size and Relationship Strength

Statistical Significance Versus Practical Significance

Key Takeaways

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Null Hypothesis Examples

What Is the Null Hypothesis?

Examples of the Null Hypothesis

Other Types of Hypotheses

Key Takeaways

User Preferences

Keyboard Shortcuts

Step 1: State the Null Hypothesis

Step 2: State the Alternative Hypothesis

Step 3: Set \(\alpha\)

Step 4: Collect Data

Step 5: Calculate a test statistic

Step 6: Construct Acceptance / Rejection regions

Step 7: Based on Steps 5 and 6, draw a conclusion about \(H_0\)

Null hypothesis significance testing: a short tutorial