hypothesis scientific order

What Are The Steps Of The Scientific Method?

Julia Simkus

Editor at Simply Psychology

BA (Hons) Psychology, Princeton University

Julia Simkus is a graduate of Princeton University with a Bachelor of Arts in Psychology. She is currently studying for a Master's Degree in Counseling for Mental Health and Wellness in September 2023. Julia's research has been published in peer reviewed journals.

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Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

Science is not just knowledge. It is also a method for obtaining knowledge. Scientific understanding is organized into theories.

The scientific method is a step-by-step process used by researchers and scientists to determine if there is a relationship between two or more variables. Psychologists use this method to conduct psychological research, gather data, process information, and describe behaviors.

It involves careful observation, asking questions, formulating hypotheses, experimental testing, and refining hypotheses based on experimental findings.

How it is Used

The scientific method can be applied broadly in science across many different fields, such as chemistry, physics, geology, and psychology. In a typical application of this process, a researcher will develop a hypothesis, test this hypothesis, and then modify the hypothesis based on the outcomes of the experiment.

The process is then repeated with the modified hypothesis until the results align with the observed phenomena. Detailed steps of the scientific method are described below.

Keep in mind that the scientific method does not have to follow this fixed sequence of steps; rather, these steps represent a set of general principles or guidelines.

7 Steps of the Scientific Method

Psychology uses an empirical approach.

Empiricism (founded by John Locke) states that the only source of knowledge comes through our senses – e.g., sight, hearing, touch, etc.

Empirical evidence does not rely on argument or belief. Thus, empiricism is the view that all knowledge is based on or may come from direct observation and experience.

The empiricist approach of gaining knowledge through experience quickly became the scientific approach and greatly influenced the development of physics and chemistry in the 17th and 18th centuries.

Step 1: Make an Observation (Theory Construction)

Every researcher starts at the very beginning. Before diving in and exploring something, one must first determine what they will study – it seems simple enough!

By making observations, researchers can establish an area of interest. Once this topic of study has been chosen, a researcher should review existing literature to gain insight into what has already been tested and determine what questions remain unanswered.

This assessment will provide helpful information about what has already been comprehended about the specific topic and what questions remain, and if one can go and answer them.

Specifically, a literature review might implicate examining a substantial amount of documented material from academic journals to books dating back decades. The most appropriate information gathered by the researcher will be shown in the introduction section or abstract of the published study results.

The background material and knowledge will help the researcher with the first significant step in conducting a psychology study, which is formulating a research question.

This is the inductive phase of the scientific process. Observations yield information that is used to formulate theories as explanations. A theory is a well-developed set of ideas that propose an explanation for observed phenomena.

Inductive reasoning moves from specific premises to a general conclusion. It starts with observations of phenomena in the natural world and derives a general law.

Step 2: Ask a Question

Once a researcher has made observations and conducted background research, the next step is to ask a scientific question. A scientific question must be defined, testable, and measurable.

A useful approach to develop a scientific question is: “What is the effect of…?” or “How does X affect Y?”

To answer an experimental question, a researcher must identify two variables: the independent and dependent variables.

The independent variable is the variable manipulated (the cause), and the dependent variable is the variable being measured (the effect).

An example of a research question could be, “Is handwriting or typing more effective for retaining information?” Answering the research question and proposing a relationship between the two variables is discussed in the next step.

Step 3: Form a Hypothesis (Make Predictions)

A hypothesis is an educated guess about the relationship between two or more variables. A hypothesis is an attempt to answer your research question based on prior observation and background research. Theories tend to be too complex to be tested all at once; instead, researchers create hypotheses to test specific aspects of a theory.

For example, a researcher might ask about the connection between sleep and educational performance. Do students who get less sleep perform worse on tests at school?

It is crucial to think about different questions one might have about a particular topic to formulate a reasonable hypothesis. It would help if one also considered how one could investigate the causalities.

It is important that the hypothesis is both testable against reality and falsifiable. This means that it can be tested through an experiment and can be proven wrong.

The falsification principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory to be considered scientific, it must be able to be tested and conceivably proven false.

To test a hypothesis, we first assume that there is no difference between the populations from which the samples were taken. This is known as the null hypothesis and predicts that the independent variable will not influence the dependent variable.

Examples of “if…then…” Hypotheses:

• If one gets less than 6 hours of sleep, then one will do worse on tests than if one obtains more rest.
• If one drinks lots of water before going to bed, one will have to use the bathroom often at night.
• If one practices exercising and lighting weights, then one’s body will begin to build muscle.

The research hypothesis is often called the alternative hypothesis and predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Although one could state and write a scientific hypothesis in many ways, hypotheses are usually built like “if…then…” statements.

Step 4: Run an Experiment (Gather Data)

The next step in the scientific method is to test your hypothesis and collect data. A researcher will design an experiment to test the hypothesis and gather data that will either support or refute the hypothesis.

The exact research methods used to examine a hypothesis depend on what is being studied. A psychologist might utilize two primary forms of research, experimental research, and descriptive research.

The scientific method is objective in that researchers do not let preconceived ideas or biases influence the collection of data and is systematic in that experiments are conducted in a logical way.

Experimental Research

Experimental research is used to investigate cause-and-effect associations between two or more variables. This type of research systematically controls an independent variable and measures its effect on a specified dependent variable.

Experimental research involves manipulating an independent variable and measuring the effect(s) on the dependent variable. Repeating the experiment multiple times is important to confirm that your results are accurate and consistent.

One of the significant advantages of this method is that it permits researchers to determine if changes in one variable cause shifts in each other.

While experiments in psychology typically have many moving parts (and can be relatively complex), an easy investigation is rather fundamental. Still, it does allow researchers to specify cause-and-effect associations between variables.

Most simple experiments use a control group, which involves those who do not receive the treatment, and an experimental group, which involves those who do receive the treatment.

An example of experimental research would be when a pharmaceutical company wants to test a new drug. They give one group a placebo (control group) and the other the actual pill (experimental group).

Descriptive Research

Descriptive research is generally used when it is challenging or even impossible to control the variables in question. Examples of descriptive analysis include naturalistic observation, case studies , and correlation studies .

One example of descriptive research includes phone surveys that marketers often use. While they typically do not allow researchers to identify cause and effect, correlational studies are quite common in psychology research. They make it possible to spot associations between distinct variables and measure the solidity of those relationships.

Step 5: Analyze the Data and Draw Conclusions

Once a researcher has designed and done the investigation and collected sufficient data, it is time to inspect this gathered information and judge what has been found. Researchers can summarize the data, interpret the results, and draw conclusions based on this evidence using analyses and statistics.

Upon completion of the experiment, you can collect your measurements and analyze the data using statistics. Based on the outcomes, you will either reject or confirm your hypothesis.

Analyze the Data

So, how does a researcher determine what the results of their study mean? Statistical analysis can either support or refute a researcher’s hypothesis and can also be used to determine if the conclusions are statistically significant.

When outcomes are said to be “statistically significant,” it is improbable that these results are due to luck or chance. Based on these observations, investigators must then determine what the results mean.

An experiment will support a hypothesis in some circumstances, but sometimes it fails to be truthful in other cases.

What occurs if the developments of a psychology investigation do not endorse the researcher’s hypothesis? It does mean that the study was worthless. Simply because the findings fail to defend the researcher’s hypothesis does not mean that the examination is not helpful or instructive.

This kind of research plays a vital role in supporting scientists in developing unexplored questions and hypotheses to investigate in the future. After decisions have been made, the next step is to communicate the results with the rest of the scientific community.

This is an integral part of the process because it contributes to the general knowledge base and can assist other scientists in finding new research routes to explore.

If the hypothesis is not supported, a researcher should acknowledge the experiment’s results, formulate a new hypothesis, and develop a new experiment.

We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist that could refute a theory.

Draw Conclusions and Interpret the Data

When the empirical observations disagree with the hypothesis, a number of possibilities must be considered. It might be that the theory is incorrect, in which case it needs altering, so it fully explains the data.

Alternatively, it might be that the hypothesis was poorly derived from the original theory, in which case the scientists were expecting the wrong thing to happen.

It might also be that the research was poorly conducted, or used an inappropriate method, or there were factors in play that the researchers did not consider. This will begin the process of the scientific method again.

If the hypothesis is supported, the researcher can find more evidence to support their hypothesis or look for counter-evidence to strengthen their hypothesis further.

In either scenario, the researcher should share their results with the greater scientific community.

Step 6: Share Your Results

One of the final stages of the research cycle involves the publication of the research. Once the report is written, the researcher(s) may submit the work for publication in an appropriate journal.

Usually, this is done by writing up a study description and publishing the article in a professional or academic journal. The studies and conclusions of psychological work can be seen in peer-reviewed journals such as  Developmental Psychology , Psychological Bulletin, the  Journal of Social Psychology, and numerous others.

Scientists should report their findings by writing up a description of their study and any subsequent findings. This enables other researchers to build upon the present research or replicate the results.

As outlined by the American Psychological Association (APA), there is a typical structure of a journal article that follows a specified format. In these articles, researchers:

• Supply a brief narrative and background on previous research
• Give their hypothesis
• Specify who participated in the study and how they were chosen
• Provide operational definitions for each variable
• Explain the measures and methods used to collect data
• Describe how the data collected was interpreted
• Discuss what the outcomes mean

A detailed record of psychological studies and all scientific studies is vital to clearly explain the steps and procedures used throughout the study. So that other researchers can try this experiment too and replicate the results.

The editorial process utilized by academic and professional journals guarantees that each submitted article undergoes a thorough peer review to help assure that the study is scientifically sound. Once published, the investigation becomes another piece of the current puzzle of our knowledge “base” on that subject.

This last step is important because all results, whether they supported or did not support the hypothesis, can contribute to the scientific community. Publication of empirical observations leads to more ideas that are tested against the real world, and so on. In this sense, the scientific process is circular.

The editorial process utilized by academic and professional journals guarantees that each submitted article undergoes a thorough peer review to help assure that the study is scientifically sound.

Once published, the investigation becomes another piece of the current puzzle of our knowledge “base” on that subject.

By replicating studies, psychologists can reduce errors, validate theories, and gain a stronger understanding of a particular topic.

Step 7: Repeat the Scientific Method (Iteration)

Now, if one’s hypothesis turns out to be accurate, find more evidence or find counter-evidence. If one’s hypothesis is false, create a new hypothesis or try again.

One may wish to revise their first hypothesis to make a more niche experiment to design or a different specific question to test.

The amazingness of the scientific method is that it is a comprehensive and straightforward process that scientists, and everyone, can utilize over and over again.

So, draw conclusions and repeat because the scientific method is never-ending, and no result is ever considered perfect.

The scientific method is a process of:

• Making an observation.
• Forming a hypothesis.
• Making a prediction.
• Experimenting to test the hypothesis.

The procedure of repeating the scientific method is crucial to science and all fields of human knowledge.

Further Information

• Karl Popper – Falsification
• Thomas – Kuhn Paradigm Shift
• Positivism in Sociology: Definition, Theory & Examples
• Is Psychology a Science?
• Psychology as a Science (PDF)

List the 6 steps of the scientific methods in order

• Make an observation (theory construction)
• Ask a question. A scientific question must be defined, testable, and measurable.
• Form a hypothesis (make predictions)
• Run an experiment to test the hypothesis (gather data)
• Analyze the data and draw conclusions
• Share your results so that other researchers can make new hypotheses

What is the first step of the scientific method?

The first step of the scientific method is making an observation. This involves noticing and describing a phenomenon or group of phenomena that one finds interesting and wishes to explain.

Observations can occur in a natural setting or within the confines of a laboratory. The key point is that the observation provides the initial question or problem that the rest of the scientific method seeks to answer or solve.

What is the scientific method?

The scientific method is a step-by-step process that investigators can follow to determine if there is a causal connection between two or more variables.

Psychologists and other scientists regularly suggest motivations for human behavior. On a more casual level, people judge other people’s intentions, incentives, and actions daily.

While our standard assessments of human behavior are subjective and anecdotal, researchers use the scientific method to study psychology objectively and systematically.

All utilize a scientific method to study distinct aspects of people’s thinking and behavior. This process allows scientists to analyze and understand various psychological phenomena, but it also provides investigators and others a way to disseminate and debate the results of their studies.

The outcomes of these studies are often noted in popular media, which leads numerous to think about how or why researchers came to the findings they did.

Why Use the Six Steps of the Scientific Method

The goal of scientists is to understand better the world that surrounds us. Scientific research is the most critical tool for navigating and learning about our complex world.

Without it, we would be compelled to rely solely on intuition, other people’s power, and luck. We can eliminate our preconceived concepts and superstitions through methodical scientific research and gain an objective sense of ourselves and our world.

All psychological studies aim to explain, predict, and even control or impact mental behaviors or processes. So, psychologists use and repeat the scientific method (and its six steps) to perform and record essential psychological research.

So, psychologists focus on understanding behavior and the cognitive (mental) and physiological (body) processes underlying behavior.

In the real world, people use to understand the behavior of others, such as intuition and personal experience. The hallmark of scientific research is evidence to support a claim.

Scientific knowledge is empirical, meaning it is grounded in objective, tangible evidence that can be observed repeatedly, regardless of who is watching.

The scientific method is crucial because it minimizes the impact of bias or prejudice on the experimenter. Regardless of how hard one tries, even the best-intentioned scientists can’t escape discrimination. can’t

It stems from personal opinions and cultural beliefs, meaning any mortal filters data based on one’s experience. Sadly, this “filtering” process can cause a scientist to favor one outcome over another.

For an everyday person trying to solve a minor issue at home or work, succumbing to these biases is not such a big deal; in fact, most times, it is important.

But in the scientific community, where results must be inspected and reproduced, bias or discrimination must be avoided.

When to Use the Six Steps of the Scientific Method ?

One can use the scientific method anytime, anywhere! From the smallest conundrum to solving global problems, it is a process that can be applied to any science and any investigation.

Even if you are not considered a “scientist,” you will be surprised to know that people of all disciplines use it for all kinds of dilemmas.

Try to catch yourself next time you come by a question and see how you subconsciously or consciously use the scientific method.

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Steps of the Scientific Method 2

The scientific method is a system scientists and other people use to ask and answer questions about the natural world. In a nutshell, the scientific method works by making observations, asking a question or identifying a problem, and then designing and analyzing an experiment to test a prediction of what you expect will happen. It’s a powerful analytical tool because once you draw conclusions, you may be able to answer a question and make predictions about future events.

These are the steps of the scientific method:

• Make observations.

Sometimes this step is omitted in the list, but you always make observations before asking a question, whether you recognize it or not. You always have some background information about a topic. However, it’s a good idea to be systematic about your observations and to record them in a lab book or another way. Often, these initial observations can help you identify a question. Later on, this information may help you decide on another area of investigation of a topic.

• Ask a question, identify a problem, or state an objective.

There are various forms of this step. Sometimes you may want to state an objective and a problem and then phrase it in the form of a question. The reason it’s good to state a question is because it’s easiest to design an experiment to answer a question. A question helps you form a hypothesis, which focuses your study.

• Research the topic.

You should conduct background research on your topic to learn as much as you can about it. This can occur both before and after you state an objective and form a hypothesis. In fact, you may find yourself researching the topic throughout the entire process.

• Formulate a hypothesis.

A hypothesis is a formal prediction. There are two forms of a hypothesis that are particularly easy to test. One is to state the hypothesis as an “if, then” statement. An example of an if-then hypothesis is: “If plants are grown under red light, then they will be taller than plants grown under white light.” Another good type of hypothesis is what is called a “ null hypothesis ” or “no difference” hypothesis. An example of a null hypothesis is: “There is no difference in the rate of growth of plants grown under red light compared with plants grown under white light.”

• Design and perform an experiment to test the hypothesis.

Once you have a hypothesis, you need to find a way to test it. This involves an experiment . There are many ways to set up an experiment. A basic experiment contains variables, which are factors you can measure. The two main variables are the independent variable (the one you control or change) and the dependent variable (the one you measure to see if it is affected when you change the independent variable).

• Record and analyze the data you obtain from the experiment.

It’s a good idea to record notes alongside your data, stating anything unusual or unexpected. Once you have the data, draw a chart, table, or graph to present your results. Next, analyze the results to understand what it all means.

• Determine whether you accept or reject the hypothesis.

Do the results support the hypothesis or not? Keep in mind, it’s okay if the hypothesis is not supported, especially if you are testing a null hypothesis. Sometimes excluding an explanation answers your question! There is no “right” or “wrong” here. However, if you obtain an unexpected result, you might want to perform another experiment.

• Draw a conclusion and report the results of the experiment.

What good is knowing something if you keep it to yourself? You should report the outcome of the experiment, even if it’s just in a notebook. What did you learn from the experiment?

How Many Steps Are There?

You may be asked to list the 5 steps of the scientific method or the 6 steps of the method or some other number. There are different ways of grouping together the steps outlined here, so it’s a good idea to learn the way an instructor wants you to list the steps. No matter how many steps there are, the order is always the same.

Related Posts

2 thoughts on “ steps of the scientific method ”.

You raise a valid point, but peer review has its limitations. Consider the case of Galileo, for example.

That’s a good point too. But that was a rare limitation due to religion, and scientific consensus prevailed in the end. It’s nowhere near a reason to doubt scientific consensus in general. I’m thinking about issues such as climate change where so many people are skeptical despite 97% consensus among climate scientists. I was just surprised to see that this is not included as an important part of the process.

Comments are closed.

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• University of Nevada, Reno - College of Agriculture, Biotechnology and Natural Resources Extension - The Scientific Method
• World History Encyclopedia - Scientific Method
• LiveScience - What Is Science?
• Verywell Mind - Scientific Method Steps in Psychology Research
• WebMD - What is the Scientific Method?
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• National Center for Biotechnology Information - PubMed Central - Redefining the scientific method: as the use of sophisticated scientific methods that extend our mind
• Khan Academy - The scientific method
• Simply Psychology - What are the steps in the Scientific Method?
• Stanford Encyclopedia of Philosophy - Scientific Method

scientific method , mathematical and experimental technique employed in the sciences . More specifically, it is the technique used in the construction and testing of a scientific hypothesis .

The process of observing, asking questions, and seeking answers through tests and experiments is not unique to any one field of science. In fact, the scientific method is applied broadly in science, across many different fields. Many empirical sciences, especially the social sciences , use mathematical tools borrowed from probability theory and statistics , together with outgrowths of these, such as decision theory , game theory , utility theory, and operations research . Philosophers of science have addressed general methodological problems, such as the nature of scientific explanation and the justification of induction .

The scientific method is critical to the development of scientific theories , which explain empirical (experiential) laws in a scientifically rational manner. In a typical application of the scientific method, a researcher develops a hypothesis , tests it through various means, and then modifies the hypothesis on the basis of the outcome of the tests and experiments. The modified hypothesis is then retested, further modified, and tested again, until it becomes consistent with observed phenomena and testing outcomes. In this way, hypotheses serve as tools by which scientists gather data. From that data and the many different scientific investigations undertaken to explore hypotheses, scientists are able to develop broad general explanations, or scientific theories.

See also Mill’s methods ; hypothetico-deductive method .

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

Prevent plagiarism. Run a free check.

Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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• v.34(45); 2019 Nov 25

Scientific Hypotheses: Writing, Promoting, and Predicting Implications

Armen yuri gasparyan.

1 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, West Midlands, UK.

Lilit Ayvazyan

2 Department of Medical Chemistry, Yerevan State Medical University, Yerevan, Armenia.

Ulzhan Mukanova

3 Department of Surgical Disciplines, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

George D. Kitas

5 Arthritis Research UK Epidemiology Unit, University of Manchester, Manchester, UK.

Scientific hypotheses are essential for progress in rapidly developing academic disciplines. Proposing new ideas and hypotheses require thorough analyses of evidence-based data and predictions of the implications. One of the main concerns relates to the ethical implications of the generated hypotheses. The authors may need to outline potential benefits and limitations of their suggestions and target widely visible publication outlets to ignite discussion by experts and start testing the hypotheses. Not many publication outlets are currently welcoming hypotheses and unconventional ideas that may open gates to criticism and conservative remarks. A few scholarly journals guide the authors on how to structure hypotheses. Reflecting on general and specific issues around the subject matter is often recommended for drafting a well-structured hypothesis article. An analysis of influential hypotheses, presented in this article, particularly Strachan's hygiene hypothesis with global implications in the field of immunology and allergy, points to the need for properly interpreting and testing new suggestions. Envisaging the ethical implications of the hypotheses should be considered both by authors and journal editors during the writing and publishing process.

INTRODUCTION

We live in times of digitization that radically changes scientific research, reporting, and publishing strategies. Researchers all over the world are overwhelmed with processing large volumes of information and searching through numerous online platforms, all of which make the whole process of scholarly analysis and synthesis complex and sophisticated.

Current research activities are diversifying to combine scientific observations with analysis of facts recorded by scholars from various professional backgrounds. 1 Citation analyses and networking on social media are also becoming essential for shaping research and publishing strategies globally. 2 Learning specifics of increasingly interdisciplinary research studies and acquiring information facilitation skills aid researchers in formulating innovative ideas and predicting developments in interrelated scientific fields.

Arguably, researchers are currently offered more opportunities than in the past for generating new ideas by performing their routine laboratory activities, observing individual cases and unusual developments, and critically analyzing published scientific facts. What they need at the start of their research is to formulate a scientific hypothesis that revisits conventional theories, real-world processes, and related evidence to propose new studies and test ideas in an ethical way. 3 Such a hypothesis can be of most benefit if published in an ethical journal with wide visibility and exposure to relevant online databases and promotion platforms.

Although hypotheses are crucially important for the scientific progress, only few highly skilled researchers formulate and eventually publish their innovative ideas per se . Understandably, in an increasingly competitive research environment, most authors would prefer to prioritize their ideas by discussing and conducting tests in their own laboratories or clinical departments, and publishing research reports afterwards. However, there are instances when simple observations and research studies in a single center are not capable of explaining and testing new groundbreaking ideas. Formulating hypothesis articles first and calling for multicenter and interdisciplinary research can be a solution in such instances, potentially launching influential scientific directions, if not academic disciplines.

The aim of this article is to overview the importance and implications of infrequently published scientific hypotheses that may open new avenues of thinking and research.

Despite the seemingly established views on innovative ideas and hypotheses as essential research tools, no structured definition exists to tag the term and systematically track related articles. In 1973, the Medical Subject Heading (MeSH) of the U.S. National Library of Medicine introduced “Research Design” as a structured keyword that referred to the importance of collecting data and properly testing hypotheses, and indirectly linked the term to ethics, methods and standards, among many other subheadings.

One of the experts in the field defines “hypothesis” as a well-argued analysis of available evidence to provide a realistic (scientific) explanation of existing facts, fill gaps in public understanding of sophisticated processes, and propose a new theory or a test. 4 A hypothesis can be proven wrong partially or entirely. However, even such an erroneous hypothesis may influence progress in science by initiating professional debates that help generate more realistic ideas. The main ethical requirement for hypothesis authors is to be honest about the limitations of their suggestions. 5

EXAMPLES OF INFLUENTIAL SCIENTIFIC HYPOTHESES

Daily routine in a research laboratory may lead to groundbreaking discoveries provided the daily accounts are comprehensively analyzed and reproduced by peers. The discovery of penicillin by Sir Alexander Fleming (1928) can be viewed as a prime example of such discoveries that introduced therapies to treat staphylococcal and streptococcal infections and modulate blood coagulation. 6 , 7 Penicillin got worldwide recognition due to the inventor's seminal works published by highly prestigious and widely visible British journals, effective ‘real-world’ antibiotic therapy of pneumonia and wounds during World War II, and euphoric media coverage. 8 In 1945, Fleming, Florey and Chain got a much deserved Nobel Prize in Physiology or Medicine for the discovery that led to the mass production of the wonder drug in the U.S. and ‘real-world practice’ that tested the use of penicillin. What remained globally unnoticed is that Zinaida Yermolyeva, the outstanding Soviet microbiologist, created the Soviet penicillin, which turned out to be more effective than the Anglo-American penicillin and entered mass production in 1943; that year marked the turning of the tide of the Great Patriotic War. 9 One of the reasons of the widely unnoticed discovery of Zinaida Yermolyeva is that her works were published exclusively by local Russian (Soviet) journals.

The past decades have been marked by an unprecedented growth of multicenter and global research studies involving hundreds and thousands of human subjects. This trend is shaped by an increasing number of reports on clinical trials and large cohort studies that create a strong evidence base for practice recommendations. Mega-studies may help generate and test large-scale hypotheses aiming to solve health issues globally. Properly designed epidemiological studies, for example, may introduce clarity to the hygiene hypothesis that was originally proposed by David Strachan in 1989. 10 David Strachan studied the epidemiology of hay fever in a cohort of 17,414 British children and concluded that declining family size and improved personal hygiene had reduced the chances of cross infections in families, resulting in epidemics of atopic disease in post-industrial Britain. Over the past four decades, several related hypotheses have been proposed to expand the potential role of symbiotic microorganisms and parasites in the development of human physiological immune responses early in life and protection from allergic and autoimmune diseases later on. 11 , 12 Given the popularity and the scientific importance of the hygiene hypothesis, it was introduced as a MeSH term in 2012. 13

Hypotheses can be proposed based on an analysis of recorded historic events that resulted in mass migrations and spreading of certain genetic diseases. As a prime example, familial Mediterranean fever (FMF), the prototype periodic fever syndrome, is believed to spread from Mesopotamia to the Mediterranean region and all over Europe due to migrations and religious prosecutions millennia ago. 14 Genetic mutations spearing mild clinical forms of FMF are hypothesized to emerge and persist in the Mediterranean region as protective factors against more serious infectious diseases, particularly tuberculosis, historically common in that part of the world. 15 The speculations over the advantages of carrying the MEditerranean FeVer (MEFV) gene are further strengthened by recorded low mortality rates from tuberculosis among FMF patients of different nationalities living in Tunisia in the first half of the 20th century. 16

Diagnostic hypotheses shedding light on peculiarities of diseases throughout the history of mankind can be formulated using artefacts, particularly historic paintings. 17 Such paintings may reveal joint deformities and disfigurements due to rheumatic diseases in individual subjects. A series of paintings with similar signs of pathological conditions interpreted in a historic context may uncover mysteries of epidemics of certain diseases, which is the case with Ruben's paintings depicting signs of rheumatic hands and making some doctors to believe that rheumatoid arthritis was common in Europe in the 16th and 17th century. 18

WRITING SCIENTIFIC HYPOTHESES

There are author instructions of a few journals that specifically guide how to structure, format, and make submissions categorized as hypotheses attractive. One of the examples is presented by Med Hypotheses , the flagship journal in its field with more than four decades of publishing and influencing hypothesis authors globally. However, such guidance is not based on widely discussed, implemented, and approved reporting standards, which are becoming mandatory for all scholarly journals.

Generating new ideas and scientific hypotheses is a sophisticated task since not all researchers and authors are skilled to plan, conduct, and interpret various research studies. Some experience with formulating focused research questions and strong working hypotheses of original research studies is definitely helpful for advancing critical appraisal skills. However, aspiring authors of scientific hypotheses may need something different, which is more related to discerning scientific facts, pooling homogenous data from primary research works, and synthesizing new information in a systematic way by analyzing similar sets of articles. To some extent, this activity is reminiscent of writing narrative and systematic reviews. As in the case of reviews, scientific hypotheses need to be formulated on the basis of comprehensive search strategies to retrieve all available studies on the topics of interest and then synthesize new information selectively referring to the most relevant items. One of the main differences between scientific hypothesis and review articles relates to the volume of supportive literature sources ( Table 1 ). In fact, hypothesis is usually formulated by referring to a few scientific facts or compelling evidence derived from a handful of literature sources. 19 By contrast, reviews require analyses of a large number of published documents retrieved from several well-organized and evidence-based databases in accordance with predefined search strategies. 20 , 21 , 22

The format of hypotheses, especially the implications part, may vary widely across disciplines. Clinicians may limit their suggestions to the clinical manifestations of diseases, outcomes, and management strategies. Basic and laboratory scientists analysing genetic, molecular, and biochemical mechanisms may need to view beyond the frames of their narrow fields and predict social and population-based implications of the proposed ideas. 23

Advanced writing skills are essential for presenting an interesting theoretical article which appeals to the global readership. Merely listing opposing facts and ideas, without proper interpretation and analysis, may distract the experienced readers. The essence of a great hypothesis is a story behind the scientific facts and evidence-based data.

ETHICAL IMPLICATIONS

The authors of hypotheses substantiate their arguments by referring to and discerning rational points from published articles that might be overlooked by others. Their arguments may contradict the established theories and practices, and pose global ethical issues, particularly when more or less efficient medical technologies and public health interventions are devalued. The ethical issues may arise primarily because of the careless references to articles with low priorities, inadequate and apparently unethical methodologies, and concealed reporting of negative results. 24 , 25

Misinterpretation and misunderstanding of the published ideas and scientific hypotheses may complicate the issue further. For example, Alexander Fleming, whose innovative ideas of penicillin use to kill susceptible bacteria saved millions of lives, warned of the consequences of uncontrolled prescription of the drug. The issue of antibiotic resistance had emerged within the first ten years of penicillin use on a global scale due to the overprescription that affected the efficacy of antibiotic therapies, with undesirable consequences for millions. 26

The misunderstanding of the hygiene hypothesis that primarily aimed to shed light on the role of the microbiome in allergic and autoimmune diseases resulted in decline of public confidence in hygiene with dire societal implications, forcing some experts to abandon the original idea. 27 , 28 Although that hypothesis is unrelated to the issue of vaccinations, the public misunderstanding has resulted in decline of vaccinations at a time of upsurge of old and new infections.

A number of ethical issues are posed by the denial of the viral (human immunodeficiency viruses; HIV) hypothesis of acquired Immune deficiency Syndrome (AIDS) by Peter Duesberg, who overviewed the links between illicit recreational drugs and antiretroviral therapies with AIDS and refuted the etiological role of HIV. 29 That controversial hypothesis was rejected by several journals, but was eventually published without external peer review at Med Hypotheses in 2010. The publication itself raised concerns of the unconventional editorial policy of the journal, causing major perturbations and more scrutinized publishing policies by journals processing hypotheses.

WHERE TO PUBLISH HYPOTHESES

Although scientific authors are currently well informed and equipped with search tools to draft evidence-based hypotheses, there are still limited quality publication outlets calling for related articles. The journal editors may be hesitant to publish articles that do not adhere to any research reporting guidelines and open gates for harsh criticism of unconventional and untested ideas. Occasionally, the editors opting for open-access publishing and upgrading their ethics regulations launch a section to selectively publish scientific hypotheses attractive to the experienced readers. 30 However, the absence of approved standards for this article type, particularly no mandate for outlining potential ethical implications, may lead to publication of potentially harmful ideas in an attractive format.

A suggestion of simultaneously publishing multiple or alternative hypotheses to balance the reader views and feedback is a potential solution for the mainstream scholarly journals. 31 However, that option alone is hardly applicable to emerging journals with unconventional quality checks and peer review, accumulating papers with multiple rejections by established journals.

A large group of experts view hypotheses with improbable and controversial ideas publishable after formal editorial (in-house) checks to preserve the authors' genuine ideas and avoid conservative amendments imposed by external peer reviewers. 32 That approach may be acceptable for established publishers with large teams of experienced editors. However, the same approach can lead to dire consequences if employed by nonselective start-up, open-access journals processing all types of articles and primarily accepting those with charged publication fees. 33 In fact, pseudoscientific ideas arguing Newton's and Einstein's seminal works or those denying climate change that are hardly testable have already found their niche in substandard electronic journals with soft or nonexistent peer review. 34

CITATIONS AND SOCIAL MEDIA ATTENTION

The available preliminary evidence points to the attractiveness of hypothesis articles for readers, particularly those from research-intensive countries who actively download related documents. 35 However, citations of such articles are disproportionately low. Only a small proportion of top-downloaded hypotheses (13%) in the highly prestigious Med Hypotheses receive on average 5 citations per article within a two-year window. 36

With the exception of a few historic papers, the vast majority of hypotheses attract relatively small number of citations in a long term. 36 Plausible explanations are that these articles often contain a single or only a few citable points and that suggested research studies to test hypotheses are rarely conducted and reported, limiting chances of citing and crediting authors of genuine research ideas.

A snapshot analysis of citation activity of hypothesis articles may reveal interest of the global scientific community towards their implications across various disciplines and countries. As a prime example, Strachan's hygiene hypothesis, published in 1989, 10 is still attracting numerous citations on Scopus, the largest bibliographic database. As of August 28, 2019, the number of the linked citations in the database is 3,201. Of the citing articles, 160 are cited at least 160 times ( h -index of this research topic = 160). The first three citations are recorded in 1992 and followed by a rapid annual increase in citation activity and a peak of 212 in 2015 ( Fig. 1 ). The top 5 sources of the citations are Clin Exp Allergy (n = 136), J Allergy Clin Immunol (n = 119), Allergy (n = 81), Pediatr Allergy Immunol (n = 69), and PLOS One (n = 44). The top 5 citing authors are leading experts in pediatrics and allergology Erika von Mutius (Munich, Germany, number of publications with the index citation = 30), Erika Isolauri (Turku, Finland, n = 27), Patrick G Holt (Subiaco, Australia, n = 25), David P. Strachan (London, UK, n = 23), and Bengt Björksten (Stockholm, Sweden, n = 22). The U.S. is the leading country in terms of citation activity with 809 related documents, followed by the UK (n = 494), Germany (n = 314), Australia (n = 211), and the Netherlands (n = 177). The largest proportion of citing documents are articles (n = 1,726, 54%), followed by reviews (n = 950, 29.7%), and book chapters (n = 213, 6.7%). The main subject areas of the citing items are medicine (n = 2,581, 51.7%), immunology and microbiology (n = 1,179, 23.6%), and biochemistry, genetics and molecular biology (n = 415, 8.3%).

Interestingly, a recent analysis of 111 publications related to Strachan's hygiene hypothesis, stating that the lack of exposure to infections in early life increases the risk of rhinitis, revealed a selection bias of 5,551 citations on Web of Science. 37 The articles supportive of the hypothesis were cited more than nonsupportive ones (odds ratio adjusted for study design, 2.2; 95% confidence interval, 1.6–3.1). A similar conclusion pointing to a citation bias distorting bibliometrics of hypotheses was reached by an earlier analysis of a citation network linked to the idea that β-amyloid, which is involved in the pathogenesis of Alzheimer disease, is produced by skeletal muscle of patients with inclusion body myositis. 38 The results of both studies are in line with the notion that ‘positive’ citations are more frequent in the field of biomedicine than ‘negative’ ones, and that citations to articles with proven hypotheses are too common. 39

Social media channels are playing an increasingly active role in the generation and evaluation of scientific hypotheses. In fact, publicly discussing research questions on platforms of news outlets, such as Reddit, may shape hypotheses on health-related issues of global importance, such as obesity. 40 Analyzing Twitter comments, researchers may reveal both potentially valuable ideas and unfounded claims that surround groundbreaking research ideas. 41 Social media activities, however, are unevenly distributed across different research topics, journals and countries, and these are not always objective professional reflections of the breakthroughs in science. 2 , 42

Scientific hypotheses are essential for progress in science and advances in healthcare. Innovative ideas should be based on a critical overview of related scientific facts and evidence-based data, often overlooked by others. To generate realistic hypothetical theories, the authors should comprehensively analyze the literature and suggest relevant and ethically sound design for future studies. They should also consider their hypotheses in the context of research and publication ethics norms acceptable for their target journals. The journal editors aiming to diversify their portfolio by maintaining and introducing hypotheses section are in a position to upgrade guidelines for related articles by pointing to general and specific analyses of the subject, preferred study designs to test hypotheses, and ethical implications. The latter is closely related to specifics of hypotheses. For example, editorial recommendations to outline benefits and risks of a new laboratory test or therapy may result in a more balanced article and minimize associated risks afterwards.

Not all scientific hypotheses have immediate positive effects. Some, if not most, are never tested in properly designed research studies and never cited in credible and indexed publication outlets. Hypotheses in specialized scientific fields, particularly those hardly understandable for nonexperts, lose their attractiveness for increasingly interdisciplinary audience. The authors' honest analysis of the benefits and limitations of their hypotheses and concerted efforts of all stakeholders in science communication to initiate public discussion on widely visible platforms and social media may reveal rational points and caveats of the new ideas.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Gasparyan AY, Yessirkepov M, Kitas GD.
• Methodology: Gasparyan AY, Mukanova U, Ayvazyan L.
• Writing - original draft: Gasparyan AY, Ayvazyan L, Yessirkepov M.
• Writing - review & editing: Gasparyan AY, Yessirkepov M, Mukanova U, Kitas GD.

Scientific Method: Step 3: HYPOTHESIS

• Step 1: QUESTION
• Step 2: RESEARCH
• Step 3: HYPOTHESIS
• Step 4: EXPERIMENT
• Step 5: DATA
• Step 6: CONCLUSION

Step 3: State your hypothesis

Now it's time to state your hypothesis . The hypothesis is an educated guess as to what will happen during your experiment. 

The hypothesis is often written using the words "IF" and "THEN." For example, " If I do not study, then I will fail the test." The "if' and "then" statements reflect your independent and dependent variables . 

The hypothesis should relate back to your original question and must be testable .

A word about variables...

Your experiment will include variables to measure and to explain any cause and effect. Below you will find some useful links describing the different types of variables.

• "What are independent and dependent variables" NCES
• [VIDEO] Biology: Independent vs. Dependent Variables (Nucleus Medical Media) Video explaining independent and dependent variables, with examples.

Resource Links

• What is and How to Write a Good Hypothesis in Research? (Elsevier)
• Hypothesis brochure from Penn State/Berks

• << Previous: Step 2: RESEARCH
• Next: Step 4: EXPERIMENT >>
• Last Updated: Aug 26, 2024 10:04 AM
• URL: https://harford.libguides.com/scientific_method

Scientific Hypothesis, Model, Theory, and Law

Understanding the Difference Between Basic Scientific Terms

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• Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
• B.A., Physics and Mathematics, Hastings College

Words have precise meanings in science. For example, "theory," "law," and "hypothesis" don't all mean the same thing. Outside of science, you might say something is "just a theory," meaning it's a supposition that may or may not be true. In science, however, a theory is an explanation that generally is accepted to be true. Here's a closer look at these important, commonly misused terms.

A hypothesis is an educated guess, based on observation. It's a prediction of cause and effect. Usually, a hypothesis can be supported or refuted through experimentation or more observation. A hypothesis can be disproven but not proven to be true.

Example: If you see no difference in the cleaning ability of various laundry detergents, you might hypothesize that cleaning effectiveness is not affected by which detergent you use. This hypothesis can be disproven if you observe a stain is removed by one detergent and not another. On the other hand, you cannot prove the hypothesis. Even if you never see a difference in the cleanliness of your clothes after trying 1,000 detergents, there might be one more you haven't tried that could be different.

Scientists often construct models to help explain complex concepts. These can be physical models like a model volcano or atom  or conceptual models like predictive weather algorithms. A model doesn't contain all the details of the real deal, but it should include observations known to be valid.

Example: The  Bohr model shows electrons orbiting the atomic nucleus, much the same way as the way planets revolve around the sun. In reality, the movement of electrons is complicated but the model makes it clear that protons and neutrons form a nucleus and electrons tend to move around outside the nucleus.

A scientific theory summarizes a hypothesis or group of hypotheses that have been supported with repeated testing. A theory is valid as long as there is no evidence to dispute it. Therefore, theories can be disproven. Basically, if evidence accumulates to support a hypothesis, then the hypothesis can become accepted as a good explanation of a phenomenon. One definition of a theory is to say that it's an accepted hypothesis.

Example: It is known that on June 30, 1908, in Tunguska, Siberia, there was an explosion equivalent to the detonation of about 15 million tons of TNT. Many hypotheses have been proposed for what caused the explosion. It was theorized that the explosion was caused by a natural extraterrestrial phenomenon , and was not caused by man. Is this theory a fact? No. The event is a recorded fact. Is this theory, generally accepted to be true, based on evidence to-date? Yes. Can this theory be shown to be false and be discarded? Yes.

A scientific law generalizes a body of observations. At the time it's made, no exceptions have been found to a law. Scientific laws explain things but they do not describe them. One way to tell a law and a theory apart is to ask if the description gives you the means to explain "why." The word "law" is used less and less in science, as many laws are only true under limited circumstances.

Example: Consider Newton's Law of Gravity . Newton could use this law to predict the behavior of a dropped object but he couldn't explain why it happened.

As you can see, there is no "proof" or absolute "truth" in science. The closest we get are facts, which are indisputable observations. Note, however, if you define proof as arriving at a logical conclusion, based on the evidence, then there is "proof" in science. Some work under the definition that to prove something implies it can never be wrong, which is different. If you're asked to define the terms hypothesis, theory, and law, keep in mind the definitions of proof and of these words can vary slightly depending on the scientific discipline. What's important is to realize they don't all mean the same thing and cannot be used interchangeably.

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Theories, Hypotheses, and Laws: Definitions, examples, and their roles in science

by Anthony Carpi, Ph.D., Anne E. Egger, Ph.D.

Listen to this reading

Did you know that the idea of evolution had been part of Western thought for more than 2,000 years before Charles Darwin was born? Like many theories, the theory of evolution was the result of the work of many different scientists working in different disciplines over a period of time.

A scientific theory is an explanation inferred from multiple lines of evidence for some broad aspect of the natural world and is logical, testable, and predictive.

As new evidence comes to light, or new interpretations of existing data are proposed, theories may be revised and even change; however, they are not tenuous or speculative.

A scientific hypothesis is an inferred explanation of an observation or research finding; while more exploratory in nature than a theory, it is based on existing scientific knowledge.

A scientific law is an expression of a mathematical or descriptive relationship observed in nature.

Imagine yourself shopping in a grocery store with a good friend who happens to be a chemist. Struggling to choose between the many different types of tomatoes in front of you, you pick one up, turn to your friend, and ask her if she thinks the tomato is organic . Your friend simply chuckles and replies, "Of course it's organic!" without even looking at how the fruit was grown. Why the amused reaction? Your friend is highlighting a simple difference in vocabulary. To a chemist, the term organic refers to any compound in which hydrogen is bonded to carbon. Tomatoes (like all plants) are abundant in organic compounds – thus your friend's laughter. In modern agriculture, however, organic has come to mean food items grown or raised without the use of chemical fertilizers, pesticides, or other additives.

So who is correct? You both are. Both uses of the word are correct, though they mean different things in different contexts. There are, of course, lots of words that have more than one meaning (like bat , for example), but multiple meanings can be especially confusing when two meanings convey very different ideas and are specific to one field of study.

• Scientific theories

The term theory also has two meanings, and this double meaning often leads to confusion. In common language, the term theory generally refers to speculation or a hunch or guess. You might have a theory about why your favorite sports team isn't playing well, or who ate the last cookie from the cookie jar. But these theories do not fit the scientific use of the term. In science, a theory is a well-substantiated and comprehensive set of ideas that explains a phenomenon in nature. A scientific theory is based on large amounts of data and observations that have been collected over time. Scientific theories can be tested and refined by additional research , and they allow scientists to make predictions. Though you may be correct in your hunch, your cookie jar conjecture doesn't fit this more rigorous definition.

All scientific disciplines have well-established, fundamental theories . For example, atomic theory describes the nature of matter and is supported by multiple lines of evidence from the way substances behave and react in the world around us (see our series on Atomic Theory ). Plate tectonic theory describes the large scale movement of the outer layer of the Earth and is supported by evidence from studies about earthquakes , magnetic properties of the rocks that make up the seafloor , and the distribution of volcanoes on Earth (see our series on Plate Tectonic Theory ). The theory of evolution by natural selection , which describes the mechanism by which inherited traits that affect survivability or reproductive success can cause changes in living organisms over generations , is supported by extensive studies of DNA , fossils , and other types of scientific evidence (see our Charles Darwin series for more information). Each of these major theories guides and informs modern research in those fields, integrating a broad, comprehensive set of ideas.

So how are these fundamental theories developed, and why are they considered so well supported? Let's take a closer look at some of the data and research supporting the theory of natural selection to better see how a theory develops.

Comprehension Checkpoint

• The development of a scientific theory: Evolution and natural selection

The theory of evolution by natural selection is sometimes maligned as Charles Darwin 's speculation on the origin of modern life forms. However, evolutionary theory is not speculation. While Darwin is rightly credited with first articulating the theory of natural selection, his ideas built on more than a century of scientific research that came before him, and are supported by over a century and a half of research since.

• The Fixity Notion: Linnaeus

Figure 1: Cover of the 1760 edition of Systema Naturae .

Research about the origins and diversity of life proliferated in the 18th and 19th centuries. Carolus Linnaeus , a Swedish botanist and the father of modern taxonomy (see our module Taxonomy I for more information), was a devout Christian who believed in the concept of Fixity of Species , an idea based on the biblical story of creation. The Fixity of Species concept said that each species is based on an ideal form that has not changed over time. In the early stages of his career, Linnaeus traveled extensively and collected data on the structural similarities and differences between different species of plants. Noting that some very different plants had similar structures, he began to piece together his landmark work, Systema Naturae, in 1735 (Figure 1). In Systema , Linnaeus classified organisms into related groups based on similarities in their physical features. He developed a hierarchical classification system , even drawing relationships between seemingly disparate species (for example, humans, orangutans, and chimpanzees) based on the physical similarities that he observed between these organisms. Linnaeus did not explicitly discuss change in organisms or propose a reason for his hierarchy, but by grouping organisms based on physical characteristics, he suggested that species are related, unintentionally challenging the Fixity notion that each species is created in a unique, ideal form.

• The age of Earth: Leclerc and Hutton

Also in the early 1700s, Georges-Louis Leclerc, a French naturalist, and James Hutton , a Scottish geologist, began to develop new ideas about the age of the Earth. At the time, many people thought of the Earth as 6,000 years old, based on a strict interpretation of the events detailed in the Christian Old Testament by the influential Scottish Archbishop Ussher. By observing other planets and comets in the solar system , Leclerc hypothesized that Earth began as a hot, fiery ball of molten rock, mostly consisting of iron. Using the cooling rate of iron, Leclerc calculated that Earth must therefore be at least 70,000 years old in order to have reached its present temperature.

Hutton approached the same topic from a different perspective, gathering observations of the relationships between different rock formations and the rates of modern geological processes near his home in Scotland. He recognized that the relatively slow processes of erosion and sedimentation could not create all of the exposed rock layers in only a few thousand years (see our module The Rock Cycle ). Based on his extensive collection of data (just one of his many publications ran to 2,138 pages), Hutton suggested that the Earth was far older than human history – hundreds of millions of years old.

While we now know that both Leclerc and Hutton significantly underestimated the age of the Earth (by about 4 billion years), their work shattered long-held beliefs and opened a window into research on how life can change over these very long timescales.

• Fossil studies lead to the development of a theory of evolution: Cuvier

Figure 2: Illustration of an Indian elephant jaw and a mammoth jaw from Cuvier's 1796 paper.

With the age of Earth now extended by Leclerc and Hutton, more researchers began to turn their attention to studying past life. Fossils are the main way to study past life forms, and several key studies on fossils helped in the development of a theory of evolution . In 1795, Georges Cuvier began to work at the National Museum in Paris as a naturalist and anatomist. Through his work, Cuvier became interested in fossils found near Paris, which some claimed were the remains of the elephants that Hannibal rode over the Alps when he invaded Rome in 218 BCE . In studying both the fossils and living species , Cuvier documented different patterns in the dental structure and number of teeth between the fossils and modern elephants (Figure 2) (Horner, 1843). Based on these data , Cuvier hypothesized that the fossil remains were not left by Hannibal, but were from a distinct species of animal that once roamed through Europe and had gone extinct thousands of years earlier: the mammoth. The concept of species extinction had been discussed by a few individuals before Cuvier, but it was in direct opposition to the Fixity of Species concept – if every organism were based on a perfectly adapted, ideal form, how could any cease to exist? That would suggest it was no longer ideal.

While his work provided critical evidence of extinction , a key component of evolution , Cuvier was highly critical of the idea that species could change over time. As a result of his extensive studies of animal anatomy, Cuvier had developed a holistic view of organisms , stating that the

number, direction, and shape of the bones that compose each part of an animal's body are always in a necessary relation to all the other parts, in such a way that ... one can infer the whole from any one of them ...

In other words, Cuvier viewed each part of an organism as a unique, essential component of the whole organism. If one part were to change, he believed, the organism could not survive. His skepticism about the ability of organisms to change led him to criticize the whole idea of evolution , and his prominence in France as a scientist played a large role in discouraging the acceptance of the idea in the scientific community.

• Studies of invertebrates support a theory of change in species: Lamarck

Jean Baptiste Lamarck, a contemporary of Cuvier's at the National Museum in Paris, studied invertebrates like insects and worms. As Lamarck worked through the museum's large collection of invertebrates, he was impressed by the number and variety of organisms . He became convinced that organisms could, in fact, change through time, stating that

... time and favorable conditions are the two principal means which nature has employed in giving existence to all her productions. We know that for her time has no limit, and that consequently she always has it at her disposal.

This was a radical departure from both the fixity concept and Cuvier's ideas, and it built on the long timescale that geologists had recently established. Lamarck proposed that changes that occurred during an organism 's lifetime could be passed on to their offspring, suggesting, for example, that a body builder's muscles would be inherited by their children.

As it turned out, the mechanism by which Lamarck proposed that organisms change over time was wrong, and he is now often referred to disparagingly for his "inheritance of acquired characteristics" idea. Yet despite the fact that some of his ideas were discredited, Lamarck established a support for evolutionary theory that others would build on and improve.

• Rock layers as evidence for evolution: Smith

In the early 1800s, a British geologist and canal surveyor named William Smith added another component to the accumulating evidence for evolution . Smith observed that rock layers exposed in different parts of England bore similarities to one another: These layers (or strata) were arranged in a predictable order, and each layer contained distinct groups of fossils . From this series of observations , he developed a hypothesis that specific groups of animals followed one another in a definite sequence through Earth's history, and this sequence could be seen in the rock layers. Smith's hypothesis was based on his knowledge of geological principles , including the Law of Superposition.

The Law of Superposition states that sediments are deposited in a time sequence, with the oldest sediments deposited first, or at the bottom, and newer layers deposited on top. The concept was first expressed by the Persian scientist Avicenna in the 11th century, but was popularized by the Danish scientist Nicolas Steno in the 17th century. Note that the law does not state how sediments are deposited; it simply describes the relationship between the ages of deposited sediments.

Figure 3: Engraving from William Smith's 1815 monograph on identifying strata by fossils.

Smith backed up his hypothesis with extensive drawings of fossils uncovered during his research (Figure 3), thus allowing other scientists to confirm or dispute his findings. His hypothesis has, in fact, been confirmed by many other scientists and has come to be referred to as the Law of Faunal Succession. His work was critical to the formation of evolutionary theory as it not only confirmed Cuvier's work that organisms have gone extinct , but it also showed that the appearance of life does not date to the birth of the planet. Instead, the fossil record preserves a timeline of the appearance and disappearance of different organisms in the past, and in doing so offers evidence for change in organisms over time.

• The theory of evolution by natural selection: Darwin and Wallace

It was into this world that Charles Darwin entered: Linnaeus had developed a taxonomy of organisms based on their physical relationships, Leclerc and Hutton demonstrated that there was sufficient time in Earth's history for organisms to change, Cuvier showed that species of organisms have gone extinct , Lamarck proposed that organisms change over time, and Smith established a timeline of the appearance and disappearance of different organisms in the geological record .

Figure 4: Title page of the 1859 Murray edition of the Origin of Species by Charles Darwin.

Charles Darwin collected data during his work as a naturalist on the HMS Beagle starting in 1831. He took extensive notes on the geology of the places he visited; he made a major find of fossils of extinct animals in Patagonia and identified an extinct giant ground sloth named Megatherium . He experienced an earthquake in Chile that stranded beds of living mussels above water, where they would be preserved for years to come.

Perhaps most famously, he conducted extensive studies of animals on the Galápagos Islands, noting subtle differences in species of mockingbird, tortoise, and finch that were isolated on different islands with different environmental conditions. These subtle differences made the animals highly adapted to their environments .

This broad spectrum of data led Darwin to propose an idea about how organisms change "by means of natural selection" (Figure 4). But this idea was not based only on his work, it was also based on the accumulation of evidence and ideas of many others before him. Because his proposal encompassed and explained many different lines of evidence and previous work, they formed the basis of a new and robust scientific theory regarding change in organisms – the theory of evolution by natural selection .

Darwin's ideas were grounded in evidence and data so compelling that if he had not conceived them, someone else would have. In fact, someone else did. Between 1858 and 1859, Alfred Russel Wallace , a British naturalist, wrote a series of letters to Darwin that independently proposed natural selection as the means for evolutionary change. The letters were presented to the Linnean Society of London, a prominent scientific society at the time (see our module on Scientific Institutions and Societies ). This long chain of research highlights that theories are not just the work of one individual. At the same time, however, it often takes the insight and creativity of individuals to put together all of the pieces and propose a new theory . Both Darwin and Wallace were experienced naturalists who were familiar with the work of others. While all of the work leading up to 1830 contributed to the theory of evolution , Darwin's and Wallace's theory changed the way that future research was focused by presenting a comprehensive, well-substantiated set of ideas, thus becoming a fundamental theory of biological research.

• Expanding, testing, and refining scientific theories
• Genetics and evolution: Mendel and Dobzhansky

Since Darwin and Wallace first published their ideas, extensive research has tested and expanded the theory of evolution by natural selection . Darwin had no concept of genes or DNA or the mechanism by which characteristics were inherited within a species . A contemporary of Darwin's, the Austrian monk Gregor Mendel , first presented his own landmark study, Experiments in Plant Hybridization, in 1865 in which he provided the basic patterns of genetic inheritance , describing which characteristics (and evolutionary changes) can be passed on in organisms (see our Genetics I module for more information). Still, it wasn't until much later that a "gene" was defined as the heritable unit.

In 1937, the Ukrainian born geneticist Theodosius Dobzhansky published Genetics and the Origin of Species , a seminal work in which he described genes themselves and demonstrated that it is through mutations in genes that change occurs. The work defined evolution as "a change in the frequency of an allele within a gene pool" ( Dobzhansky, 1982 ). These studies and others in the field of genetics have added to Darwin's work, expanding the scope of the theory .

• Evolution under a microscope: Lenski

More recently, Dr. Richard Lenski, a scientist at Michigan State University, isolated a single Escherichia coli bacterium in 1989 as the first step of the longest running experimental test of evolutionary theory to date – a true test meant to replicate evolution and natural selection in the lab.

After the single microbe had multiplied, Lenski isolated the offspring into 12 different strains , each in their own glucose-supplied culture, predicting that the genetic make-up of each strain would change over time to become more adapted to their specific culture as predicted by evolutionary theory . These 12 lines have been nurtured for over 40,000 bacterial generations (luckily bacterial generations are much shorter than human generations) and exposed to different selective pressures such as heat , cold, antibiotics, and infection with other microorganisms. Lenski and colleagues have studied dozens of aspects of evolutionary theory with these genetically isolated populations . In 1999, they published a paper that demonstrated that random genetic mutations were common within the populations and highly diverse across different individual bacteria . However, "pivotal" mutations that are associated with beneficial changes in the group are shared by all descendants in a population and are much rarer than random mutations, as predicted by the theory of evolution by natural selection (Papadopoulos et al., 1999).

• Punctuated equilibrium: Gould and Eldredge

While established scientific theories like evolution have a wealth of research and evidence supporting them, this does not mean that they cannot be refined as new information or new perspectives on existing data become available. For example, in 1972, biologist Stephen Jay Gould and paleontologist Niles Eldredge took a fresh look at the existing data regarding the timing by which evolutionary change takes place. Gould and Eldredge did not set out to challenge the theory of evolution; rather they used it as a guiding principle and asked more specific questions to add detail and nuance to the theory. This is true of all theories in science: they provide a framework for additional research. At the time, many biologists viewed evolution as occurring gradually, causing small incremental changes in organisms at a relatively steady rate. The idea is referred to as phyletic gradualism , and is rooted in the geological concept of uniformitarianism . After reexamining the available data, Gould and Eldredge came to a different explanation, suggesting that evolution consists of long periods of stability that are punctuated by occasional instances of dramatic change – a process they called punctuated equilibrium .

Like Darwin before them, their proposal is rooted in evidence and research on evolutionary change, and has been supported by multiple lines of evidence. In fact, punctuated equilibrium is now considered its own theory in evolutionary biology. Punctuated equilibrium is not as broad of a theory as natural selection . In science, some theories are broad and overarching of many concepts, such as the theory of evolution by natural selection; others focus on concepts at a smaller, or more targeted, scale such as punctuated equilibrium. And punctuated equilibrium does not challenge or weaken the concept of natural selection; rather, it represents a change in our understanding of the timing by which change occurs in organisms , and a theory within a theory. The theory of evolution by natural selection now includes both gradualism and punctuated equilibrium to describe the rate at which change proceeds.

• Hypotheses and laws: Other scientific concepts

One of the challenges in understanding scientific terms like theory is that there is not a precise definition even within the scientific community. Some scientists debate over whether certain proposals merit designation as a hypothesis or theory , and others mistakenly use the terms interchangeably. But there are differences in these terms. A hypothesis is a proposed explanation for an observable phenomenon. Hypotheses , just like theories , are based on observations from research . For example, LeClerc did not hypothesize that Earth had cooled from a molten ball of iron as a random guess; rather, he developed this hypothesis based on his observations of information from meteorites.

A scientist often proposes a hypothesis before research confirms it as a way of predicting the outcome of study to help better define the parameters of the research. LeClerc's hypothesis allowed him to use known parameters (the cooling rate of iron) to do additional work. A key component of a formal scientific hypothesis is that it is testable and falsifiable. For example, when Richard Lenski first isolated his 12 strains of bacteria , he likely hypothesized that random mutations would cause differences to appear within a period of time in the different strains of bacteria. But when a hypothesis is generated in science, a scientist will also make an alternative hypothesis , an explanation that explains a study if the data do not support the original hypothesis. If the different strains of bacteria in Lenski's work did not diverge over the indicated period of time, perhaps the rate of mutation was slower than first thought.

So you might ask, if theories are so well supported, do they eventually become laws? The answer is no – not because they aren't well-supported, but because theories and laws are two very different things. Laws describe phenomena, often mathematically. Theories, however, explain phenomena. For example, in 1687 Isaac Newton proposed a Theory of Gravitation, describing gravity as a force of attraction between two objects. As part of this theory, Newton developed a Law of Universal Gravitation that explains how this force operates. This law states that the force of gravity between two objects is inversely proportional to the square of the distance between those objects. Newton 's Law does not explain why this is true, but it describes how gravity functions (see our Gravity: Newtonian Relationships module for more detail). In 1916, Albert Einstein developed his theory of general relativity to explain the mechanism by which gravity has its effect. Einstein's work challenges Newton's theory, and has been found after extensive testing and research to more accurately describe the phenomenon of gravity. While Einstein's work has replaced Newton's as the dominant explanation of gravity in modern science, Newton's Law of Universal Gravitation is still used as it reasonably (and more simply) describes the force of gravity under many conditions. Similarly, the Law of Faunal Succession developed by William Smith does not explain why organisms follow each other in distinct, predictable ways in the rock layers, but it accurately describes the phenomenon.